Singh, MandeepGuzmán Aránguez, Ana IsabelHussain, AfzalSrinivas, Cheerneni S.Kaur, Indu P.2023-06-172023-06-172019-01-291743-588910.2217/nnm-2018-0278https://hdl.handle.net/20.500.14352/13263Aim: Evaluation of solid lipid nanoparticles (SLNs) for ocular delivery of isoniazid (INH). Materials & methods: INH-SLNs were characterized for morphological, thermal, crystalline and nuclear magnetic resonance properties. In vitro release and ex vivo corneal permeability of INH-SLNs was also evaluated. Proof-of-concept uptake studies were performed in corneal and conjunctival cell lines and in vivo in rat eye using fluorescein-labeled SLNs. Antimycobacterial activity of INH-SLNs was confirmed. In vivo aqueous humor pharmacokinetics, toxicity and tolerance was performed in rabbit/rat eye. Results: INH-SLNs showed extended release (48 h), enhanced corneal permeability (1.6-times), five-times lower MIC, significant in vitro and in vivo uptake of fluorescein-labeled SLNs, 4.2-times ocular bioavailability (area under the curve) and in vivo acute and repeat dose safety. Conclusion: INH-SLNs are an effective ocular delivery system.engjournal articlehttps://doi.org/10.2217/nnm-2018-0278https://www.futuremedicine.com/doi/abs/10.2217/nnm-2018-0278?rfr_dat=cr_pub%3Dpubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&journalCode=nnmopen access577.1616.24-00.5:617.7Cellular uptakeCorneal permeabilityCytotoxicityIn vitro releaseIn vivo ocular toxicityMinimum inhibitory concentrationMicroemulsificationOcular tissue uptakeOcular tuberculosisStability studiesBioquímica (Química)Anatomía ocular