Patiño Álvarez, Aurora BelénMedina, Á.Domenech Lucas, MiriamGonzález Jaén, María TeresaJiménez, M.Vázquez, C.2024-02-022024-02-022007-020265-203X10.1080/02652030600967222https://hdl.handle.net/20.500.14352/98309Penicillium brevicompactum is a ubiquitous fungal species that contaminates diverse substrates and commodities and produces an array of metabolites toxic to human and animals. The present work has obtained evidence, by liquid chromatography (LC)-ion-trap mass spectrometry, of the ability of P. brevicompactum strains isolated from grapes to produce mycophenolic acid, a potent immunosuppressor. In order to facilitate early diagnosis of this species on commodities for human and animal consumption, a rapid, sensitive and specific polymerase chain reaction (PCR) assay for P. brevicompactum was developed. The specific primers were designed based on the ITS1-5.8S-ITS2ITS (Internal Transcribed Spacers of rRNA genes) multicopy region. This method provides a useful aid to detect the presence of this fungal species in grapes and other commodities in order to prevent the toxins produced entering the food chain.engjournal article1464–5122https://www.tandfonline.com/doi/full/10.1080/02652030600967222restricted access579663.1Penicillium brevicompactumMycophenolic acidPolymerase chain reaction (PCR)GrapesLiquid chromatography (LC)-ion-trap mass spectrometryMicrobiología (Biología)Tecnología de los alimentos3309.90 Microbiología de Alimentos