Luque Uría, ÁlvaroPeltomaa, RiikkaNavarro Duro, MarinaFikacek, SabrinaHead, TrajenDeo, Sapna K.Daunert, SylviaBenito Peña, María ElenaMoreno Bondi, María Cruz2023-06-222023-06-222022-02-172629-274210.1002/anse.202100070https://hdl.handle.net/20.500.14352/71361CRUE-CSIC (Acuerdos Transformativos 2022)The development of two different immunoassays for the determination of fumonisin B1 in wheat samples is reported. A previously described mimopeptide for fumonisin B1 (FB1) was used to produce fusion proteins in combination with two different luciferases: Gaussia luciferase (GLuc) and NanoLuc luciferase (NLuc). The production, expression and the development of two immunoassays based on these fusion proteins (A2- GLuc and A2-NLuc) is detailed. The assay showing the best performance, A2-NLuc, with a limit of detection of 0.61 ngmL 1 and a dynamic range from 1.9 to 95 ngmL 1 , was employed for the analysis of spiked wheat samples, a reference matrix material, as well as naturally contaminated wheat samples. The recoveries obtained in the spiked samples were acceptable, between 81.5 and 109%, with relative standard deviations lower than 14%. The analysis of naturally contaminated wheat was validated by a liquid chromatography coupled to tandem mass detection method.engAtribución-NoComercial-SinDerivadas 3.0 Españajournal articlehttps://doi.org/10.1002/anse.202100070open access543bioluminescencefumonisin B1fusion proteinsimmunoassaysluciferasesQuímica analítica (Química)2301 Química Analítica