Jemni Damer , NahlaGuedan Duran , AtochaCichy, JasminLozano Picazo, PalomaGonzalez Nieto , DanielPerez Rigueiro, JoséRojo, FranciscoGuinea, Gustavo V.Virtuoso , AssuntaCirillo, GIovanniPapa, MicheleArmada Maresca, FelixLargo Aramburu , CarlotaD Aznar Cervantes, SalvadorCenis, José L.Panetsos Petrova, Fivos2024-01-092024-01-092020Jemni-Damer, Nahla, et al. «First steps for the development of silk fibroin-based 3D biohybrid retina for age-related macular degeneration (AMD)». Journal of Neural Engineering, vol. 17, n.o 5, octubre de 2020, p. 055003. https://doi.org/10.1088/1741-2552/abb9c0.1741-255210.1088/1741-2552/abb9c0https://hdl.handle.net/20.500.14352/92056Age-related macular degeneration is an incurable chronic neurodegenerative disease, causing progressive loss of the central vision and even blindness. Up-to-date therapeutic approaches can only slow down he progression of the disease. Objective. Feasibility study for a multilayered, silk fibroin-based, 3D biohybrid retina. Approach. Fabrication of silk fibroin-based biofilms; culture of different types of cells: retinal pigment epithelium, retinal neurons, Müller and mesenchymal stem cells ; creation of a layered structure glued with silk fibroin hydrogel. Main results. In vitro evidence for the feasibility of layered 3D biohybrid retinas; primary culture neurons grow and develop neurites on silk fibroin biofilms, either alone or in presence of other cells cultivated on the same biomaterial; cell organization and cellular phenotypes are maintained in vitro for the seven days of the experiment. Significance. 3D biohybrid retina can be built using silk silkworm fibroin films and hydrogels to be used in cell replacement therapy for AMD and similar retinal neurodegenerative diseases.engjournal articlehttps://doi.org/10.1088/1741-2552/abb9c0https://iopscience.iop.org/article/10.1088/1741-2552/abb9c0restricted access612.8ScaffoldsRetinal pigment epitheliumNeuronsCultured cellsIn vitroMacular degenerationNeurociencias (Biológicas)2490 Neurociencias