Santiago, BegoñaGalindo Izquierdo, MaríaRivero, MiguelBrehmer, M.Mateo, I.Pablos Álvarez, José Luis2025-01-142025-01-142002-07-01Santiago B, Galindo M, Rivero M, Brehmer MT, Mateo I, Pablos JL. The chemoattraction of lymphocytes by rheumatoid arthritis - synovial fluid is not dependent on the chemokine receptor CCR5. Rheumatol Int. 2002 Jul;22(3):107-11.0172-817210.1007/s00296-002-0203-1https://hdl.handle.net/20.500.14352/114228Objective: The objective was to study the potential role of the chemokine receptor CCR5 in the chemoattraction of lymphocytes by rheumatoid arthritis synovial fluid (RA-SF). Methods: The expression of the CCR5 receptor was studied by flow cytometry. Chemotaxis of peripheral blood lymphocytes in response to RA-SF was analyzed on transmigration chambers. Chemotaxis of immortalized lymphocytes from individuals homozygous for the Delta32 deletion of the CCR5 gene (CCR5-/-) was analyzed. The effect of a neutralizing anti-CCR5 antibody on the migration of CCR5+/+ cells was also studied. Results: We confirmed an increase in the proportion of CCR5-expressing lymphocytes in RA-SF and a preferential migration of CCR5+ lymphocytes toward RA-SF in vitro. CCR5-/- lymphocytes showed decreased chemotactic responses to the chemokine MIP-1beta but not to RA-SF. The chemotactic responses of CCR5+/+ lymphocytes to RA-SF were not modified by anti-CCR5 neutralizing antibody. Conclusions: We confirm a preferential accumulation of CCR5-expressing lymphocytes into RA-SF. However, the chemotactic responses of lymphocytes to RA-SF were not dependent on a functional CCR5 receptor, suggesting that CCR5 is a marker of a lymphocyte subset rather than a specific mediator of chemotactic responses to chemokines in RA-SF.engjournal article1437-160Xhttps://doi.org/10.1007/s00296-002-0203-1https://link.springer.com/article/10.1007/s00296-002-0203-1restricted access616.72-022.77Rheumatoid arthritisChemokinesChemotaxisLymphocytesSynovial fluidT cellsCiencias BiomédicasReumatología32 Ciencias Médicas3205.09 Reumatología