Pianeta, RoquelinaIniesta Albentosa, Margarita IsabelCastillo, Diana MarcelaLafaurie, Gloria InésSanz Alonso, MarianoHerrera González, David2024-12-202024-12-202021-09-12Pianeta R, Iniesta M, Castillo DM, Lafaurie GI, Sanz M, Herrera D. Characterization of the Subgingival Cultivable Microbiota in Patients with Different Stages of Periodontitis in Spain and Colombia. A Cross-Sectional Study. Microorganisms. 2021 Sep 12;9(9):1940. doi: 10.3390/microorganisms909194010.3390/microorganisms9091940https://hdl.handle.net/20.500.14352/113127The objective was to characterize and compare the subgingival microbiota in patients diagnosed according to the World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions 2018. For this cross-sectional study, Spanish and Colombian subjects (characterized as health/gingivitis, periodontitis in stages I-II or stages III-IV) were clinically assessed, and subgingival samples were taken and processed by culture. The comparisons among patients with periodontal status (and between countries) was made using Mann-Whitney, Kruskal-Wallis, ANOVA and chi-square tests. The final sample consisted of 167 subjects. Eikenella corrodens and Parvimonas micra were more frequently detected in health/gingivitis and Porphyromonas gingivalis in periodontitis (p < 0.05). Higher total counts were observed in Colombia (p = 0.036). In Spain, significantly higher levels of P. gingivalis and Campylobacter rectus were observed, and of Tannerella forsythia, P. micra, Prevotella intermedia, Fusobacterium nucleatum, Actinomyces odontolyticus and Capnocytophaga spp. in Colombia (p < 0.001). P. micra was more prevalent in health/gingivitis and stage I-II periodontitis in Colombia, and P. gingivalis in all periodontitis groups in Spain (p < 0.05). As conclusions, significant differences were detected in the microbiota between health/gingivitis and periodontitis, with minor differences between stages of periodontitis. Differences were also relevant between countries, with Colombia showing larger counts and variability of bacterial species.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal article2076-2607https://doi.org/10.3390/microorganisms909194034576835https://www.mdpi.com/2076-2607/9/9/1940https://pubmed.ncbi.nlm.nih.gov/34576835/open access579.61(460+861)616.314.17-008.1(460+861)ColombiaSpainMicrobiological culturePeriodontitisSubgingival microbiotaPeriodonciaMicrobiología médica3213.13 Ortodoncia-Estomatología3201.03 Microbiología Clínica