Pérez Sancho, MartaDurán-Ferrer, ManuelGarcía-Seco Romero, María TeresaMacías, PaulaGarcía Benzaquén, NereaMartínez Alares, IreneRuiz, ElenaLegaz, EmilioDíez Guerrier, Alberto AntoineGonzález Domínguez, SergioDomínguez Rodríguez, Lucas JoséÁlvarez Sánchez, Julio2024-01-162024-01-162014Pérez-Sancho, Marta, et al. «Interferon-Gamma Responses in Sheep Exposed to Virulent and Attenuated Brucella Melitensis Strains». Veterinary Immunology and Immunopathology, vol. 160, n.o 1-2, julio de 2014, pp. 123-28. https://doi.org/10.1016/j.vetimm.2014.03.014.0165-242710.1016/j.vetimm.2014.03.014https://hdl.handle.net/20.500.14352/93254Antibody detection is the basis of large-scale sheep brucellosis diagnosis because of its sensitivity and specificity. In contrast, information on the cellular mediated immune (CMI) response triggered after Brucella melitensis infection, a cornerstone in the protection against this pathogen, is more limited, particularly regarding the effect of the virulence of the infecting strain in the induced CMI reaction. Here, the interferon-gamma (IFN-gamma) profiles evoked after exposure by different routes to virulent (H38) and attenuated (Rev.1) B. melitensis strains in 14 pregnant sheep and 87 ewe lambs, respectively, were characterized accounting for different host-related factors, and compared with their serological response and with the basal IFN-gamma responses observed in 155 animals non exposed to Brucella. No significant differences in the IFN-gamma response of Rev.1 vaccinated animals depending on the inoculation route was observed, in contrast with their serological results. Response in H38- challenged followed a similar trend although peaked later, and an effect of the abortion on the IFN-gamma response was detected. This information could help to understand the interaction bacteria–host that leads to its intracellular survival and could be useful for the design of new diagnostic approaches.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://doi.org/10.1016/j.vetimm.2014.03.014restricted access636.09IFN-gammaBrucella melitensisRev.1SheepImmunologyCell mediated immune responseInmunología veterinariaVeterinaria3109 Ciencias Veterinarias