Clemente, Luis FelipeHernáez, María LuisaRamos-Fernández, AntonioLigero, GertrudisGil, ConchaCorrales, Fernando JoséMarcilla, Miguel2023-06-172023-06-172018-07-051535-3907https://hdl.handle.net/20.500.14352/13379Currently, 14% of the human proteome is made up of proteins whose existence is not confirmed by mass spectrometry. We performed a proteomic profiling of human mesenchymal stem cells derived from adipose tissue or umbilical cord (PRIDE accession number: PXD009893) and identified peptides derived from 13 of such missing proteins. Remarkably, we found compelling evidence of the expression of hyaluronan synthase 1 (NX_Q92839-1) and confirmed its identification by the fragmentation of four heavy-labeled peptides that coeluted with their endogenous light counterparts. Our data also suggest that mesenchymal stem cells constitute a promising source for the detection of missing proteins.engjournal articleopen access579Microbiología (Farmacia)3302.03 Microbiología Industrial