Salazar Corral, Juan JoséSatriano, AndreaMatamoros, José A.Fernández Albarral, JoséGarcía Martín, Elena SalobrarLópez Cuenca, InésHoz Montañana, María Rosa DeSánchez-Puebla Fernández, LidiaRamírez Sebastián, José ManuelAlonso, CristinaSatta, ValentinaHernández Fisac, InésSagredo Ezquioga, OnintzaRamírez Sebastián, Ana Isabel2023-06-222023-06-222023-02-01Salazar, Juan J., Satriano, A., Matamoros, J. A. et al. «Retinal Tissue Shows Glial Changes in a Dravet Syndrome Knock-in Mouse Model». International Journal of Molecular Sciences, vol. 24, n.o 3, febrero de 2023, p. 2727. DOI.org (Crossref), https://doi.org/10.3390/ijms24032727.1422-006710.3390/ijms24032727https://hdl.handle.net/20.500.14352/72989Dravet syndrome (DS) is an epileptic encephalopathy caused by mutations in the Scn1a gene encoding the α1 subunit of the Nav1.1 sodium channel, which is associated with recurrent and generalized seizures, even leading to death. In experimental models of DS, histological alterations have been found in the brain; however, the retina is a projection of the brain and there are no studies that analyze the possible histological changes that may occur in the disease. This study analyzes the retinal histological changes in glial cells (microglia and astrocytes), retinal ganglion cells (RGCs) and GABAergic amacrine cells in an experimental model of DS (Syn-Cre/Scn1aWT/A1783V) compared to a control group at postnatal day (PND) 25. Retinal whole-mounts were labeled with anti-GFAP, anti-Iba-1, anti-Brn3a and anti-GAD65/67. Signs of microglial and astroglial activation, and the number of Brn3a+ and GAD65+67+ cells were quantified. We found retinal activation of astroglial and microglial cells but not death of RGCs and GABAergic amacrine cells. These changes are similar to those found at the level of the hippocampus in the same experimental model in PND25, indicating a relationship between brain and retinal changes in DS. This suggests that the retina could serve as a possible biomarker in DS.engAttribution 4.0 Internationaljournal articlehttps://doi.org/10.3390/ijms24032727https://www.mdpi.com/1422-0067/24/3/2727open access616-056.7611.8.018.24617.735Dravet syndromeRetinaAstrocytesMicrogliaRetinal ganglion cellsGABAergic amacrine cellsSyn-Cre/Scn1aWT/A1783V miceNeurociencias (Medicina)OftalmologíaAnatomía ocular2490 Neurociencias3201.09 Oftalmología