López Vázquez, AnaTorrado Maya, AlejandroHervás, ManuelNavarro, José. A.Reyes Sosa, Francisco M.Díez, BrunoMolina Heredia, Fernando P.2026-04-212026-04-212016-05-25Ana López Vázquez, Alejandro Torrado, Manuel Hervás, José A. Navarro, Francisco M. Reyes-Sosa, Bruno Díez, Fernando P. Molina-Heredia, Improving enzyme cocktails for lignocellulose hydrolysis in biorefineries by rational protein design, New Biotechnology, Volume 33, Issue 3, 2016, Page 410, ISSN 1871-6784, https://doi.org/10.1016/j.nbt.2015.10.067. (https://www.sciencedirect.com/science/article/pii/S1871678415002368)1871-678410.1016/j.nbt.2015.10.067https://hdl.handle.net/20.500.14352/134911Plant biomass provides an abundant source of sustainable energy and chemical building blocks, mainly in the form of carbohydrates, which can be used in the newly established biorefineries through the release of fermentable sugars. Fermentation of these sugars can produce valuable commercial end products such as biofuels (i.e. bioethanol) and biochemicals. The improvement of enzymatic reactions to hydrolyze biomass to fermentable sugars is essential to improve the profitability of the process. An area of research directed at reducing costs and improving the yield of biofuel production processes is focused on improving the technical efficiency of the individual enzymes, or of the whole enzyme cocktails used to generate fermentable sugars from biomass. Using protein-engineering techniques, we have designed structural and functional mutants of different cellulolytic enzymes. Rational mutants have been designed through evolutionary trace studies, based on similar functions and relevant characteristics to acquire enhanced stability in specific culture conditions. This process is becoming a powerful tool for the rational design of engineered enzymes.engconference output1876-434710.1016/j.nbt.2015.10.067https://www.sciencedirect.com/science/article/pii/S1871678415002368open access581.1577.15620.9560Fisiología vegetal (Biología)Bioquímica (Biología)Biotecnología2417.19 Fisiología Vegetal2403 Bioquímica3101 Agroquímica2302.09 Enzimología