Moya-Cavas, TamaraNavarro Villoslada, FernandoUrraca Ruiz, JavierAntonio Serrano, LuisOrellana Moraleda, GuillermoMoreno-Bondi, María CruzMoreno Bondi, María Cruz2024-01-082024-01-082023Moya-Cavas, T.; Navarro-Villoslada, F.; Lucas Urraca, J.; Antonio Serrano, L.; Orellana, G.; Cruz Moreno-Bondi, M. Simultaneous determination of zearalenone and alternariol mycotoxins in oil samples using mixed molecularly imprinted polymer beads. Food Chem. 2023, 412, 135538 DOI:10.1016/j.foodchem.2023.135538.0308-814610.1016/j.foodchem.2023.135538https://hdl.handle.net/20.500.14352/91822This work reports the optimization of a method using Molecularly Imprinted Polymers (MIPs) for the simultaneous determination of zearalenone and alternariol mycotoxins. The method was optimized using a chemometric approach where in the optimized conditions, the cartridges with a mixture (50:50, w/w) of both MIPs, were loaded with 30 mL of sample, washed with 2 mL of ACN/water (20/80, v/v) and eluted with 2.5 mL of trifluoroacetic acid/MeOH (3/97, v/v). The extracts were analyzed by HPLC coupled to a fluorescence detector (FLD). The optimized method has been applied and validated to the analysis of the mycotoxins in maize, sunflower and olive oils samples with a limit of detection of 5 and 2 μg kg- 1, respectively. Recoveries were in the range of 94 % to 108 % (RSD < 6 %) for zearalenone and 92 % to 113 % (RSD < 5 %) for alternariol. The results were confirmed by HPLC-MS/MS.engjournal articlehttps://doi.org/10.1016/j.foodchem.2023.135538https://www.sciencedirect.com/science/article/pii/S0308814623001541?via%3Dihubopen access543AlternariolMolecularly imprinted polymerMycotoxinSolid Phase ExtractionZearalenoneQuímica analítica (Química)2301.03 Análisis Cromatográfico