López Duarte, IsmaelMarkéta KubánkováDarya KiryushkoMarina K. Kuimova2025-01-202025-01-202018Kubánková M, López-Duarte I, Kiryushko D, Kuimova MK. Molecular rotors report on changes in live cell plasma membrane microviscosity upon interaction with beta-amyloid aggregates. Soft Matter 2018;14:9466–74. https://doi.org/10.1039/C8SM01633J.1744-683X1744-684810.1039/c8sm01633jhttps://hdl.handle.net/20.500.14352/115177Amyloid deposits of aggregated beta-amyloid A(1-42) peptides are a pathological hallmark of Alzheimer's disease. A(1-42) aggregates are known to induce biophysical alterations in cells, including disruption of plasma membranes. We investigated the microviscosity of plasma membranes upon interaction with oligomeric and fibrillar forms of A(1-42). Viscosity-sensing fluorophores termed molecular rotors were utilised to directly measure the microviscosities of giant plasma membrane vesicles (GPMVs) and plasma membranes of live SH-SY5Y and HeLa cells. The fluorescence lifetimes of membrane-inserting BODIPY-based molecular rotors revealed a decrease in bilayer microviscosity upon incubation with A(1-42) oligomers, while fibrillar A(1-42) did not significantly affect the microviscosity of the bilayer. In addition, we demonstrate that the neuroprotective peptide H3 counteracts the microviscosity change induced by A(1-42) oligomers, suggesting the utility of H3 as a neuroprotective therapeutic agent in neurodegenerative disorders and indicating that ligand-induced membrane stabilisation may be a possible mechanism of neuroprotection during neurodegenerative disorders such as Alzheimer's disease.engjournal articlehttps://doi.org/10.1039/C8SM01633Jrestricted access577.1612.015612.8Molecular rotorsPlasma membraneBeta-amyloid aggregatesMicroviscosityNeurodegenerative diseasesNeurociencias (Farmacia)Bioquímica (Farmacia)23 Química