Vaz, Catarina OliveiraReales Calderón, José AntonioPitarch Velasco, AídaVellosillo, PercevalTrevisan Herraz, MarcoHernáez Sánchez, Maria LuisaMonteoliva Díaz, LucíaGil García, Concha2023-06-172023-06-172019-04-15Vaz, C. O., Reales Calderón, J. A., Pitarch Velasco, A. et al. «Enrichment of ATP Binding Proteins Unveils Proteomic Alterations in Human Macrophage Cell Death, Inflammatory Response, and Protein Synthesis after Interaction with Candida Albicans». Journal of Proteome Research, vol. 18, n.o 5, mayo de 2019, pp. 2139-59. DOI.org (Crossref), https://doi.org/10.1021/acs.jproteome.9b00032.1535-389310.1021/acs.jproteome.9b00032https://hdl.handle.net/20.500.14352/13404Macrophages are involved in the primary human response to Candida albicans. After pathogen recognition, signaling pathways are activated, leading to the production of cytokines, chemokines, and antimicrobial peptides. ATP binding proteins are crucial for this regulation. Here, a quantitative proteomic and phosphoproteomic approach was carried out for the study of human macrophage ATP-binding proteins after interaction with C. albicans. From a total of 547 nonredundant quantified proteins, 137 were ATP binding proteins and 59 were detected as differentially abundant. From the differentially abundant ATP-binding proteins, 6 were kinases (MAP2K2, SYK, STK3, MAP3K2, NDKA, and SRPK1), most of them involved in signaling pathways. Furthermore, 85 phosphopeptides were quantified. Macrophage proteomic alterations including an increase of protein synthesis with a consistent decrease in proteolysis were observed. Besides, macrophages showed changes in proteins of endosomal trafficking together with mitochondrial proteins, including some involved in the response to oxidative stress. Regarding cell death mechanisms, an increase of antiapoptotic over pro-apoptotic signals is suggested. Furthermore, a high pro-inflammatory response was detected, together with no upregulation of key mi-RNAs involved in the negative feedback of this response. These findings illustrate a strategy to deepen the knowledge of the complex interactions between the host and the clinically important pathogen C. albicans.engjournal articlehttps//doi.org/10.1021/acs.jproteome.9b00032https://pubs.acs.org/doi/10.1021/acs.jproteome.9b00032open access582.282:612.017577.112579MacrophagesCandida albicansProteomicsSILACATP binding proteinsBiología molecular (Biología)Bioquímica (Biología)Microbiología (Biología)Microbiología (Farmacia)2415 Biología Molecular2302 Bioquímica2414 Microbiología3302.03 Microbiología Industrial