Arcos Navarrete, DanielGómez Cerezo, María NatividadSaiz-Pardo Sanz, MelchorPablo Velasco, David deOrtega Medina, LuisEnciso, SilviaFernández Tomé, BlancaDíaz Güemes, IdoiaSánchez Margallo, Francisco MiguelCasarrubios Palomar, LuisFeito Castellano, María JoséPortolés Pérez, María TeresaVallet Regí, María Dulce Nombre2023-06-222023-06-222022-08-031742-706110.1016/j.actbio.2022.07.067https://hdl.handle.net/20.500.14352/72100CRUE-CSIC (Acuerdos Transformativos 2022) RESEARCHER ID L-6167-2014 (Daniel Arcos Navarrete) ORCID 0000-0002-5367-7272 (Daniel Arcos Navarrete) RESEARCHER ID U-1678-2017 (María Teresa Portolés Pérez) ORCID 0000-0002-9681-0184 (María Teresa Portolés Pérez) RESEARCHER ID M-3378-2014 (María Vallet Regí) ORCID 0000-0002-6104-4889 (María Vallet Regí)The osteogenic capability of mesoporous bioactive nanoparticles (MBNPs) in the SiO2–CaO system has been assessed in vivo using an osteoporotic rabbit model. MBNPs have been prepared using a double template method, resulting in spherical nanoparticles with a porous core-shell structure that has a high surface area and the ability to incorporate the anti-osteoporotic drug ipriflavone. In vitro expression of the pro-inflammatory genes NF-κB1, IL-6, TNF-α, P38 and NOS2 in RAW-264.7 macrophages, indicates that these nanoparticles do not show adverse inflammatory effects. An injectable system has been prepared by suspending MBNPs in a hyaluronic acid-based hydrogel, which has been injected intraosseously into cavitary bone defects in osteoporotic rabbits. The histological analyses evidenced that MBNPs promote bone regeneration with a moderate inflammatory response. The incorporation of ipriflavone into these nanoparticles resulted in a higher presence of osteoblasts and enhanced angiogenesis at the defect site, but without showing significant differences in terms of new bone formation.engAtribución-NoComercial-SinDerivadas 3.0 Españajournal articlehttps://doi.org/10.1016/j.actbio.2022.07.067open accessMesoporous bioactive nanoparticlesOsteoporosisBone regenerationIpriflavoneHyaluronic acidSistema musculoesqueléticoBioquímica (Farmacia)Química farmaceútica2411.10 Fisiología del Músculo2390 Química Farmacéutica