Nguyen, KimKubota, MilesDel Arco, JonFeng, ChaoSingha, MonikaBeasley, SamanthaSakr, JasmineGandhi, Sunil P.Blurton-Jones, MatthewFernández Lucas, JesusSpitale, Robert C.2024-10-242024-10-242020-11Nguyen K, Kubota M, Del Arco J, Feng C, Singha M, Beasley S, Sakr J, Gandhi SP, Blurton-Jones M, Fernández Lucas J, Spitale RC. A Bump-Hole Strategy for Increased Stringency of Cell-Specific Metabolic Labeling of RNA. ACS Chem Biol 2020;15:3099–105. https://doi.org/10.1021/acschembio.0c00755.1554-892910.1021/acschembio.0c00755https://hdl.handle.net/20.500.14352/109478RNA research in the Spitale lab is supported by startup funds from the University of California, Irvine, the NIH Director’s New Innovator Award (1DP2GM119164 RCS). S.B. is supported by an NIH training grant 5T32CA009054-40.Profiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, we design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for cell-specific metabolic labeling of RNA with exceptional specificity and stringency.engjournal article1554-8937https://doi.org/10.1021/acschembio.0c00755https://pubs.acs.org/doi/10.1021/acschembio.0c00755open access577.15577.2575GeneticsImaging probesLabelingPeptides and proteinsUracilBioquímica (Biología)Biología molecular (Biología)Genética2403 Bioquímica2415 Biología Molecular2302.09 Enzimología2302.04 Genética Bioquímica