Arévalo Pérez, BeatrizBlázquez García, MarinaValverde de la Fuente, AlejandroSerafín González-Carrato, VerónicaMontero Calle, AnaSolís Fernández, GuillermoBarderas Manchado, RodrigoCampuzano Ruiz, SusanaYáñez-Sedeño Orive, PalomaPingarron, José M.2023-06-222023-06-222022-03-140026-367210.1007/s00604-022-05250-4https://hdl.handle.net/20.500.14352/71579CRUE-CSIC (Acuerdos Transformativos 2022)A dual immunosensor is reported for the simultaneous determination of two important immunity-related cytokines: BAFF (B cell activation factor) and APRIL (a proliferation-induced signal). Sandwich-type immunoassays with specifc antibodies (cAbs) and a strategy for signal amplifcation based on labelling the detection antibodies (dAbs) with binary MoS2/ MWCNTs nanostructures and using horseradish peroxidase (HRP) were implemented. Amperometric detection was carried out at screen-printed dual carbon electrodes (SPdCEs) through the hydroquinone HQ/H2O2 system. The developed dual immunosensor provided limit of detection (LOD) of 0.08 and 0.06 ng mL−1 for BAFF and APRIL, respectively, and proved to be useful for the determination of both cytokines in cancer cell lysates and serum samples from patients diagnosed with autoimmune diseases and cancer. The obtained results agreed with those found using ELISA methodologies.engAtribución 3.0 Españajournal articlehttps://doi.org/10.1007/s00604-022-05250-4open access543Dual immunoplatformBAFFAPRILMoS2/MWCNTsAmperometrySerumSLECRCQuímica analítica (Química)2301 Química Analítica