Valverde de la Fuente, AlejandroMontero Calle, AnaArévalo Pérez, BeatrizSan Segundo Acosta, PabloSerafín González-Carrato, VerónicaAlonso Navarro, MirenSolís Fernández, GuillermoPingarrón Carrazón, José ManuelCampuzano Ruiz, SusanaBarderas Manchado, Rodrigo2023-06-162023-06-162021-07-272629-2742doi.org/10.1002/anse.202100024https://hdl.handle.net/20.500.14352/4685CRUE-CSIC (Acuerdos Transformativos 2021)An electrochemical biosensing platform for serum autoantibodies (AAbs) detection is reported in this work, exploiting for the first time six Alzheimer's disease (AD)-specific phage-derived and frameshift aberrant HaloTag peptides as receptors, immobilized on magnetic microbeads (MBs) surface and captured on disposable electrodes to perform amperometric detection. Operational analytical characteristics and clinical diagnostic ability of the bioplatform were probed in optimized key experimental conditions by analysing serum AAbs of AD patients and healthy subjects. The value of 100% obtained for AUC, sensitivity, and selectivity from the all peptides combined ROC curve, indicate full AD-diagnostic capability of the methodology, which was further implemented, as proof of concept, in a POC multiplexing platform to detect the signature in a single test over clinically actionable times (1 h 15 min), opening great promise for the type of diagnosis and AD patients’ monitoring follow-up currently pursued.engAtribución-NoComercial-SinDerivadas 3.0 Españajournal articlehttps://doi.org/10.1002/anse.202100024open access543Alzheimer’sdiseaseautoantibodieselectrochemical biosensorsphage-derivedpeptidesreceptorsQuímica analítica (Química)2301 Química Analítica