Ramírez Sebastián, Ana IsabelFernández Albarral, JoséHoz Montañana, María Rosa DeLópez Cuenca, InésGarcía Martín, Elena SalobrarRojas Lozano, María Del PilarValiente Soriano, Francisco JavierAvilés Trigueros, MarcelinoVillegas Pérez, María PazVidal Sanz, ManuelTriviño Casado, AlbertoSalazar Corral, Juan JoséRamírez Sebastián, José ManuelBagetta, GiacintoNucci, Carlo2023-06-172023-06-172020Ramírez Sebastián, A. I., Fernández Arrabal, J. A., Hoz Montañana, M. R. et al. «Microglial Changes in the Early Aging Stage in a Healthy Retina and an Experimental Glaucoma Model». Progress in Brain Research, vol. 256, Elsevier, 2020, pp. 125-49. DOI.org (Crossref), https://doi.org/10.1016/bs.pbr.2020.05.024.978-0-12-821106-90079-612310.1016/bs.pbr.2020.05.024https://hdl.handle.net/20.500.14352/8772Available online 16 June 2020.Glaucoma is an age-related neurodegenerative disease that begins at the onset of aging. In this disease, there is an involvement of the immune system and therefore of the microglia. The purpose of this study is to evaluate the microglial activation using a mouse model of ocular hypertension (OHT) at the onset of aging. For this purpose, we used both naive and ocular hypertensives of 15-month-old mice (early stage of aging). In the latter, we analyzed the OHT eyes and the eyes contralateral to them to compare them with their aged controls. In the eyes of aged naive, aged OHT and aged contralateral eyes, microglial changes were observed compared to the young mice, including: (i) aged naive vs young naive: An increased soma size and vertical processes; (ii) aged OHT eyes vs young OHT eyes: A decrease in the area of the retina occupied by Iba-1 cells and in vertical processes; and (iii) aged contralateral vs young contralateral: A decrease in the soma size and arbor area and an increase in the number of microglia in the outer segment layer. Aged OHT eyes and the eyes contralateral to them showed an up-regulation of the CD68 expression in the branched microglia and a down-regulation in the MHCII and P2RY12 expression with respect to the eyes of young OHT mice. Conclusion: in the early phase of aging, morphological microglial changes along with changes in the expression of MHCII, CD68 and P2RY12, in both naive and OHT mice. These changes appear in aged OHT eyes and the eyes contralateral to them eyes.engbook parthttps://doi.org/10.1016/bs.pbr.2020.05.024https://www.sciencedirect.com/science/article/pii/S0079612320300662restricted access617.7-007.681611.8.018.84617.735MicrogliaAgingGlaucomaOcular hypertensionRetinaInflammationMHCIICD68P2RY12Iba-1MouseBioquímica (Medicina)OftalmologíaAnatomía ocular3201.09 Oftalmología