Berzal, SOcaña-Salceda, CSancho, MónicaOrzaez, MMesseguer, ARuiz-Ortega, MEgido, JVicent, MJOrtiz, AlbertoRamos, Adrián. MUcero Herrería, Álvaro Conrado2025-01-282025-01-282013-01-02'Ucero AC, Berzal S, Ocaña-Salceda C, Sancho M, Orzáez M, Messeguer A, Ruiz- Ortega M, Egido J, Vicent MJ, Ortiz A, Ramos AM. A polymeric nanomedicine diminishes inflammatory events in renal tubular cells. PLoS One. 2013;8(1):e51992'.10.1371/journal.pone.0051992https://hdl.handle.net/20.500.14352/116684The polyglutamic acid/peptoid 1 (QM56) nanoconjugate inhibits apoptosis by interfering with Apaf-1 binding to procaspase-9. We now describe anti-inflammatory properties of QM56 in mouse kidney and renal cell models.In cultured murine tubular cells, QM56 inhibited the inflammatory response to Tweak, a non-apoptotic stimulus. Tweak induced MCP-1 and Rantes synthesis through JAK2 kinase and NF-κB activation. Similar to JAK2 kinase inhibitors, QM56 inhibited Tweak-induced NF-κB transcriptional activity and chemokine expression, despite failing to inhibit NF-κB-p65 nuclear translocation and NF-κB DNA binding. QM56 prevented JAK2 activation and NF-κB-p65(Ser536) phosphorylation. The anti-inflammatory effect and JAK2 inhibition by QM56 were observed in Apaf-1(-/-) cells. In murine acute kidney injury, QM56 decreased tubular cell apoptosis and kidney inflammation as measured by down-modulations of MCP-1 and Rantes mRNA expression, immune cell infiltration and activation of the JAK2-dependent inflammatory pathway.In conclusion, QM56 has an anti-inflammatory activity which is independent from its role as inhibitor of Apaf-1 and apoptosis and may have potential therapeutic relevance.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://doi.org/10.1371/journal.pone.005199223300960https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0051992open access61NanomedicineApoptosisMesotheliumCiencias Biomédicas24 Ciencias de la Vida32 Ciencias Médicas