Villalonga, AnabelSánchez Sánchez, AlfredoVilela García, DianaMayol Hornero, BeatrizMartínez Ruiz, María PalomaVillalonga Santana, Reynaldo2026-01-082026-01-082022Villalonga, Anabel, et al. «Electrochemical Aptasensor Based on Anisotropically Modified (Janus-Type) Gold Nanoparticles for Determination of C-Reactive Protein». Microchimica Acta, vol. 189, n.o 8, agosto de 2022, p. 309. Springer Link, https://doi.org/10.1007/s00604-022-05420-4.10.1007/s00604-022-05420-4https://hdl.handle.net/20.500.14352/129599Novel Janus nanoparticles based on Au colloids anisotropically modified with polyamidoamine dendrons were prepared though a masking/toposelective modification approach. These nanomaterials were further functionalized with horseradish peroxidase on the dendritic face and provided on the opposite metal surface with a ssDNA aptamer for C-reactive protein (CRP). The resulting nanoparticles were employed as biorecognition/signaling elements to construct an amperometric aptasensor with sandwich-type architecture for the specific detection of this cardiac biomarker. To do this, screen-printed carbon electrodes modified with electrodeposited Au nanoparticles and functionalized with anti-CRP aptamers were used as transduction interface. The aptasensor was employed for the amperometric detection of CRP (working potential: − 200 mV vs pseudo-Ag/AgCl) in the broad range from 10 pg·mL−1 to 1.0 ng·mL−1 with a detection limit of 3.1 pg·mL−1. This electroanalytical device also showed good specificity, reproducibility (RSD = 9.8%, n = 10), and stability and was useful to quantify CRP in reconstituted human serum samples, with a RSD of 13.3%.engjournal articlehttps://doi.org/10.1007/s00604-022-05420-4https://link.springer.com/article/10.1007/s00604-022-05420-4restricted access543AptasensorAmperometryJanus nanoparticlesC-reactive proteinAu nanoparticlesDendrimerQuímica23 Química