Castromil Benito, Estela S.Betancor, DianaParrón Ballesteros, JorgeBueno Díaz, CristinaGutiérrez Díaz, GloriaTurnay, Javierde las Heras, ManuelCuesta Herranz, JavierVillalba, MaytePastor Vargas, Carlos2024-10-302024-10-302024-07-31Castromil-Benito ES, Betancor D, Parrón-Ballesteros J, Bueno-Díaz C, Gutiérrez-Díaz G, Turnay J, Heras ML, Cuesta-Herranz J, Villalba M, Pastor-Vargas C. Walnut Jug r 1 is Responsible for Primary Sensitization among Patients Suffering Walnut-Hazelnut 2S Albumin Cross-Reactivity. J Agric Food Chem. 2024 Aug 14;72(32):18162-18170. doi: 10.1021/acs.jafc.4c0360310.1021/acs.jafc.4c03603https://hdl.handle.net/20.500.14352/109761Walnut and hazelnut coallergy is a frequent manifestation in clinical practice whose molecular basis remains unclear. For this purpose, walnut-hazelnut cross-reactivity was evaluated in 20 patients allergic to one or both tree nuts and sensitized to their 2S albumins. Immunoblotting assays showed that 85% of patients recognized Jug r 1, walnut 2S albumin, which was associated with the development of severe symptoms; 50% of them corecognized hazelnut 2S albumin, Cor a 14. Both allergens were isolated using chromatographic techniques. Inhibition ELISAs revealed that Jug r 1 strongly inhibited the binding of Cor a 14-specific IgE, but Cor a 14 only partially inhibited Jug r 1-specific IgE binding. Our results showed that patients sensitized to walnut/hazelnut 2S albumins were not a homogeneous population. There were patients sensitized to specific epitopes of walnut 2S albumins and patients sensitized to cross-reactive epitopes between walnut and hazelnut, with Jug r 1 being the primary sensitizer.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://doi.org/10.1021/acs.jafc.4c03603https://pubs.acs.org/doi/10.1021/acs.jafc.4c03603open access577.12S albuminsCor a 14Jug r 1AllergenComponent-resolved diagnosisCross-reactivityFood allergyMolecular diagnosisPrimary sensitizerSeed storage proteinsFood allergyJug r 1Cor a 14Bioquímica (Química)Alergología2302 Bioquímica