Guevara, GovindaPerera González, JesúsNavarro Llorens, Juana María2023-06-182023-06-182017978-1-4939-7182-4 (Print) 978-1-4939-7183-1 (Online)1064-3745, ESSN: 1940-602910.1007/978-1-4939-7183-1_24https://hdl.handle.net/20.500.14352/19465Thin-layer chromatography (TLC) is a useful and convenient method for the analysis of steroids due to: its simple sample preparation, low time-consuming process, high sensitivity, low equipment investment and capacity to work on many samples simultaneously. Here we describe a TLC easy protocol very useful to analyze steroid molecules derived from a biotransformation carried out in wild-type and mutant resting cells of Rhodococcus ruber strain Chol-4. Following this protocol, we were able to detect the presence or the absence of some well-known intermediates of cholesterol catabolism in Rhodococcus, namely AD, ADD, and 9OHAD.engbook parthttps://link.springer.com/protocol/10.1007/978-1-4939-7183-1_24restricted access577.175.62Resting cellsDetectionThin-layer chromatographyTLCSteroidsADADD9OHADBioquímica (Biología)2302 Bioquímica