López-Calleja, Inés MaríaCruz, Silvia de laGonzález Alonso, María IsabelGarcía Antón, María TeresaMartín, Rosario2024-01-192024-01-192015López-Calleja, Inés María, et al. «Market Analysis of Food Products for Detection of Allergenic Walnut (Juglans Regia) and Pecan (Carya Illinoinensis) by Real-Time PCR». Food Chemistry, vol. 177, junio de 2015, pp. 111-19. https://doi.org/10.1016/j.foodchem.2015.01.017.0308-814610.1016/j.foodchem.2015.01.017https://hdl.handle.net/20.500.14352/94175Two real-time polymerase chain reaction (PCR)-based assays for detection of walnut (Juglans regia) and pecan (Carya illinoinensis) traces in a wide range of processed foods are described here. The method consists on a real-time PCR assay targeting the ITS1 region, using a nuclease (TaqMan) probe labeled with FAM and BBQ. The method was positive for walnut and pecan respectively, and negative for all other heterologous plants and animals tested. Using a series of model samples with defined raw walnut in wheat flour and heat-treated walnut in wheat flour with a range of concentrations of 0.1–100,000 mg/kg, a practical detection limit of 0.1 mg/kg of walnut content was estimated. Identical binary mixtures were done for pecan, reaching the same limit of detection of 0.1 mg/kg. The assay was successfully trialed on a total of 232 commercial foodstuffs.engjournal articlehttps://doi.org/10.1016/j.foodchem.2015.01.017restricted accessTaqMan real-time PCRWalnut (Juglans regia)Pecan (Carya illinoinensis)Commercial food productsFood allergyVeterinaria31 Ciencias Agrarias