Llorente-Sáez, CeliaSerrano-López, JuliaGarcía Delicado, EsmerildaPérez Sen, RaquelGómez Villafuertes, María RosaOrtega De La O, FelipeOrtega De La O, Felipe2025-12-092025-12-092025Llorente-Sáez, C., Serrano-López, J., Delicado, E. G., Pérez-Sen, R., Gómez-Villafuertes, R., & Ortega, F. (2025). Low-Density Primary Cell Culture of Postnatal Murine Cerebellar Progenitors In Vitro. Methods in molecular biology (Clifton, N.J.), 2899, 35–46. https://doi.org/10.1007/978-1-0716-4386-0_3978107164385397810716438601064-37451940-602910.1007/978-1-0716-4386-0_3https://hdl.handle.net/20.500.14352/128627Cerebellum is the major player of motor functions of the body, as well as being involved in plenty of nonmotor behavior traits. There are numerous disorders related to cerebellum that have severe consequences for patients and the absence of an effective treatment, so it is crucial to emphasize conducting research directed to deeply understand the biology of this structure, giving special importance to stem cells that could have regenerative potential. Here, we describe a novel protocol for isolating neural stem cells from postnatal mouse cerebellum, allowing for the study of progenitor cells from three distinct proliferative niches. Cells are maintained in low-density cultures without external growth factors, facilitating the study of intrinsic programming. We also suggest numerous applications that could provide an insight into the identity, development, and behavior of progenitor cells, which may contribute to the development of treatments for cerebellar disorders.engbook parthttps://doi.org/10.1007/978-1-0716-4386-040067615https://link.springer.com/protocol/10.1007/978-1-0716-4386-0_3metadata only access57761576CerebellumNeural stem cellsProgenitorsPostnatal micePrimary cell cultureLow-density cultureIn vitroNeurociencias (Biológicas)Bioquímica (Biología)Biología molecular (Biología)Farmacología (Medicina)2490 Neurociencias2403 Bioquímica2415 Biología Molecular2302.22 Farmacología Molecular