Cuadrado Vives, María CarmenCheng, HsiaopoSanchiz Giraldo, ÁfricaBallesteros Redondo, María IsabelEasson, MichaelGrimm, CaseyDiéguez, CarmenLinacero De La Fuente, M. RosarioBurbano, CarmenMaleki, Soheila2023-06-172023-06-172018Cuadrado Vives, M. C., Cheng, H., Sanchiz Giraldo, Á. et al. «Influence of Enzymatic Hydrolysis on the Allergenic Reactivity of Processed Cashew and Pistachio». Food Chemistry, vol. 241, febrero de 2018, pp. 372-79. DOI.org (Crossref), https://doi.org/10.1016/j.foodchem.2017.08.120.0308-814610.1016/j.foodchem.2017.08.120https://hdl.handle.net/20.500.14352/12103Cashew and pistachio allergies are considered a serious health problem. Previous studies have shown that thermal processing, pressurization and enzymatic hydrolysis may reduce the allergenic properties of food by changing the protein structure. This study assesses the allergenic properties of cashew and pistachio after thermal treatment (boiling and autoclaving), with or without pressure (autoclaving), and multiple enzymatic treatments under sonication, by SDS-PAGE, western blot and ELISA, with serum IgE of allergic individuals, and mass spectroscopy. Autoclaving and enzymatic hydrolysis under sonication separately induced a measurable reduction in the IgE binding properties of pastes made from treated cashew and pistachio nuts. These treatments were more effective with pistachio allergens. However, heat combined with enzymatic digestion was necessary to markedly lower IgE binding to cashew allergens. The findings identify highly effective simultaneous processing conditions to reduce or even abolish the allergenic potency of cashew and pistachio.engjournal articlehttps://doi.org/10.1016/j.foodchem.2017.08.120https://www.sciencedirect.com/science/article/pii/S0308814617314541restricted access616.056.3:612.392577.27579.67CashewPistachioFood allergyEnzymatic hydrolysisProcessed foodsThermal processingPressure processingSonicationAlergologíaBiotecnologíaGenética3207.01 Alergias3399 Otras Especialidades Tecnológicas2409 Genética