Guerrero Irigoyen, SaraAgüí Chicharro, María LourdesYáñez-Sedeño Orive, PalomaPingarrón Carrazón, José Manuel2024-01-112024-01-112020Guerrero S, Agüí L, Yáñez-Sedeño P, Pingarrón JM. Design of electrochemical immunosensors using electro-click chemistry. Application to the detection of IL-1β cytokine in saliva. Bioelectrochemistry. 2020 Feb 14;133:107484 - 107490.1878-562X10.1016/j.bioelechem.2020.107484https://hdl.handle.net/20.500.14352/92649Electro-click methodology was employed to prepare an electrochemical immunosensor for the cytokine interleukin 1β (IL-1β). The strategy involved binding of ethynylated IgG to azide-MWCNTs modified electrodes by Cu(I) catalyzed-cycloaddition reaction where the catalyst was electrochemically synthesized. This electro-click protocol is significantly faster and greener than the methods for catalyst generation through chemical reduction. The oriented immobilization of the capture antibody onto IgG-MWCNTs conjugates allowed the preparation of a sandwich-type immunosensor using biotinylated anti-IL-1β as detector antibody labeled with alkaline phosphatase-streptavidin (AP-strept). Differential pulse voltammetric transduction through the 1-naphthylphosphate/1-naphthol system was carried out. The analytical characteristics achieved with the electrochemical immunosensor showed a calibration curve exhibiting two linear ranges between 10 and 200 pg mL−1 (r2 = 0.998), and from 200 to 1200 pg mL−1 (r2 = 0.998), and a LOD value of 5.2 pg mL−1, an improvement compared with those claimed for commercial ELISA kits. In addition, the assay time was at least one hour shorter. Excellent performance was observed in the determination of IL-1β in saliva with no need for sample treatment, and by simple interpolation using a calibration plot constructed with standard solutions of the target cytokine.engAttribution-NonCommercial 4.0 Internationaljournal article1567-5394https://doi.org/10.1016/j.bioelechem.2020.10748432087557restricted access543544.6:543.25543.25:544.6Click chemistryElectro-clickElectrochemical immunosensorInterleukin 1bIL-1bCytokineSalivaBiomarkerInflammationQuímica analítica (Química)2301 Química Analítica2301.04 Análisis Electroquímico