González Gil, AlfredoPicazo González, Rosa AnaOrtega De La O, Felipe2026-01-212026-01-212025Alfredo González-Gil and Rosa Ana Picazo (2025) Neurospheres from Ovarian Cortical Cells in Mammals: Generation In Vitro and Neurosphere Assay. In Methods of Molecular Biology, Neural Stem Cells, Chapter 6. Edited by Felipe Ortega. Ed. Springuer Nature. https://doi.org/10.1007/978-1-0716-4386-0_6,978-1-0716-4388-4978-1-0716-4386-0978-1-0716-4385-31064-374510.1007/978-1-0716-4386-0https://hdl.handle.net/20.500.14352/130741Isolation of neural stem cells/neural progenitor cells (NSC/NPC) from extra-neural origin, or adult stem cells able to specify into NSC/NPC in vitro under particular cell culture conditions, offers an interesting alternative experimental model with prospective benefits in basic research and translational medicine. In this chapter, we describe a procedure of isolation and culture of ovarian cortical cells that consistently allows the spontaneous generation of a large number of neurospheres (NS), and the methods to demonstrate that their integrating NSC/NPC self-renew and are able to differentiate into neurons and glia, during the NS assay.engbook part1940-6029https://doi.org/10.1007/978-1-0716-4386-0restricted access636.09Ovarian cortical cellsNeural stem cellsNeural progenitorsNeurospheresNeurosphere assayVeterinaria3109 Ciencias Veterinarias