Sánchez Pérez, Silvia NereaVignoletti, FabioSanz-Martin, IgnacioCoca, AlejandroSanz-Esporrin, JHernando-Pradíes, IreneSantamaría, SilviaHerrera González, DavidMaldonado Bautista, EstelaGarcia-Sanz, Jose AlbertoSanz Alonso, Mariano2024-02-092024-02-092022-03Sanchez N, Vignoletti F, Sanz-Martin I, Coca A, Nuñez J, Maldonado E, Sanz-Esporrin J, Hernando-Pradíes I, Santamaría S, Herrera D, Garcia-Sanz JA, Sanz M. Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study. Int J Mol Sci. 2022 Mar 17;23(6):3248. doi: 10.3390/ijms23063248. PMID: 35328670; PMCID: PMC8950013.10.3390/ijms23063248.https://hdl.handle.net/20.500.14352/100911(1) Background: To investigate the effect of a xenogeneic collagen matrix (CMX) seeded with autologous gingiva-derived mesenchymal cells (GMSCs) when combined with a coronally advanced flap (CAF) in the treatment of localized gingival recession type 1 (RT1). (2) Methods: Dehiscence-type defects were created in seven dogs. GMSCs were isolated, transfected with a vector carrying green fluorescent protein (GFP) and expanded. Once chronified, the defects were randomly treated with (1) CAF plus the combination of CMX and GFP+ GMSCs, (2) CAF plus CMX with autologous fibroblasts, (3) CAF plus CMX and (4) CAF alone. Histological and clinical outcomes at 2- and 6-week healing periods were analyzed and compared among groups. (3) Results: Histologically, the addition of autologous cells to the CMX resulted in reduced inflammation and a variable degree of new cementum/bone formation. CMX plus GMSCs resulted in greater mean recession reduction (1.42; SD = 1.88 mm) and percentage of teeth with recession reduction of ≥2 mm (57%) when compared to the other groups, although these differences were not statistically significant. (4) Conclusions: The histometric and clinical results indicated a positive trend favouring the combination of CMX and GMSCs with the CAF when compared to the groups without cells, although these differences were not statistically significant.engjournal articleopen access616.314.17-008.1cell therapygingiva-derived stem cellsgingival recessionmesenchymal stem cellsmesenchymal stromal cellsroot coveragesoft tissue regenerationOdontología (Odontología)Periodoncia32 Ciencias Médicas