Fernandez-Novo, AitorSantos López, SergioBarrajon-Masa, ClaraMozas, Patriciade Mercado, EduardoCaceres, ElisaGarrafa, AizicGonzález Martín, Juan VicentePerez-Villalobos, NatividadOliet, AgustínAstiz, SusanaPerez-Garnelo, Sonia2025-01-232025-01-232021Fernandez-Novo, A., Santos-Lopez, S., Barrajon-Masa, C., Mozas, P., de Mercado, E., Caceres, E., Garrafa, A., Gonzalez-Martin, J. V., Perez-Villalobos, N., Oliet, A., Astiz, S., & Perez-Garnelo, S. S. (2021). Effect of Extender, Storage Time and Temperature on Kinetic Parameters (CASA) on Bull Semen Samples. Biology, 10(8), 806. https://doi.org/10.3390/biology1008080610.3390/biology10080806https://hdl.handle.net/20.500.14352/115873Conceptualization: A.F.-N., S.S.P.-G. and S.A.; methods: All authors; software: A.F.-N., S.S.-L., C.B.-M., P.M., A.G., A.O. and S.A.; validation: A.F.-N., S.S.P.-G. and S.A.; data analysis: A.F.-N., C.B.-M., P.M., E.d.M., E.C., A.G., A.O., S.S.P.-G. and S.A.; investigation: A.F.-N., S.S.P.-G. and S.A.; resources: A.F.-N., S.S.-L., A.G., A.O., S.S.P.-G. and S.A.; data curation: A.F.-N., E.d.M., E.C., J.V.G.-M. and S.A.; drafting of the manuscript: A.F.-N., S.S.P.-G. and S.A.; review and editing of the manuscript: All authors.; supervision: A.F.-N., S.S.P.-G. and S.A.; project administration: S.S.P.-G. and S.A. All authors have read and agreed to the published version of the manuscript.CASA kinetic parameters are often evaluated in a diagnostic centre. How storage conditions affect ejaculates up to evaluation is unclear. We assessed, in 25 commercial bulls electroejaculated in the field, the impact of time until evaluation (0–2 h, 4–6 h, and 24 h post-ejaculation), holding temperature (5 ◦C vs. room temperature), and extender (AndroMed®, BIOXcell® or INRA96®) on CASA kinetic parameters. Total and progressive motility, VCL, VAP, VCL, ALH, BCF, STR, LIN, and WOB were assessed. CASA kinetic parameters were preserved for up to 4–6 h post-ejaculation, except for AndroMed®. Regardless of extender or temperature, motility decreased from 4–6 h up to 24 h, with the best values obtained with BIOXcell® at 5 ◦C. Our results suggest that BIOXcell® can preserve sperm motility for up to 6 h, either at 5 ◦C or room temperature, and also INRA96® at room temperature, with motility assessments and the percentage of the most rapid sperms being the lowest with INRA96® at 5 ◦C. The kinetic parameters decreased when analyses were performed at 24 h. Therefore, we suggest evaluating seminal quality as soon as possible, before 6 h after collection. These results help to fix adequate protocols for the short-term storage and shipment of bovine semen collected under field conditions.engjournal article2079-7737https://doi.org/10.3390/biology1008080634440041open access636.09Short-term storageSemen extenderSperm kineticsComputer assisted semen analyses (CASA)BBSEVeterinaria3109 Ciencias Veterinarias