Mejías, RaquelCosto, RocíoRoca, Alejandro G.Fernández Arias, CristinaVeintemillas-Verdaguer, S.González-Carreño, T.Puerto Morales, M. delSerna, C.J.Mañes, S.Barber, D.F.2024-01-302024-01-302008-09-100168-365910.1016/j.jconrel.2008.05.028https://hdl.handle.net/20.500.14352/96469Attachment of cytokines to magnetic nanoparticles has been developed as a system for controlled local drug release in cancer therapy. We studied the adsorption/release of murine interferon gamma (IFN-gamma) on negatively charged magnetic nanoparticles prepared by three different methods, including coprecipitation, decomposition in organic media, and laser pyrolysis. To facilitate IFN-gamma adsorption, magnetic nanoparticles were surface modified by distinct molecules to achieve high negative charge at pH 7, maintaining small aggregate size and stability in biological media. We analyzed carboxylate-based coatings and studied the colloidal properties of the resulting dispersions. Finally, we incubated the magnetic dispersions with IFN-gamma and determined optimal conditions for protein adsorption onto the particles, as well as the release capacity at different pH and as a function of time. Particles prepared by decomposition in organic media and further modified with dimercaptosuccinic acid showed the most efficient adsorption/release capacity. IFN-gamma adsorbed on these nanoparticles would allow concentration of this protein or other biomolecules at specific sites for treatment of cancer or other diseases.engjournal article1873-4995https://www.sciencedirect.com/science/article/abs/pii/S0168365908003301?via%3Dihubrestricted accessCiencias24 Ciencias de la Vida2412 Inmunología