Disposable amperometric biotool for peanut detection in processed foods by targeting a chloroplast DNA marker

Gamella Carballo, MaríaBallesteros Redondo, María IsabelRuiz Valdepeñas Montiel, VíctorSánchiz, AfricaCuadrado, CarmenPingarrón Carrazón, José ManuelLinacero De La Fuente, M. RosarioCampuzano Ruiz, Susana2024-12-052024-12-052024Gamella M, Ballesteros MI, Ruiz-Valdepeñas Montiel V, Sánchiz A, Cuadrado C, Pingarrón JM, Linacero R, Campuzano S. Disposable amperometric biotool for peanut detection in processed foods by targeting a chloroplast DNA marker. Talanta 2024;277:126350. https://doi.org/10.1016/j.talanta.2024.126350.0039-914010.1016/j.talanta.2024.126350https://hdl.handle.net/20.500.14352/112109Acknowledgements: The financial support of Grants AGL2017-83082-R, PID2021-122942OB-I00 and PID2022-136351OB-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe” is gratefully acknowledged.This work reports the development and application of a disposable amperometric sensor built on magnetic microcarriers coupled to an Express PCR strategy to amplify a specific DNA fragment of the chloroplast trnH-psbA. The procedure involves the selective capture of a 68-mer synthetic target DNA (or unmodified PCR products) through sandwich hybridization with RNA capture probe-modified streptavidin MBs and RNA signaling probes, labeled using antibodies specific to the heteroduplexes and secondary antibodies tagged with horseradish peroxidase. Amperometric measurements were performed on screen-printed electrodes using the H2O2/hydroquinone system. Achieving a LOD of 3 pM for the synthetic target, it was possible to detect 2.5 pg of peanut DNA and around 10 mg kg−1 of peanut in binary mixtures (defatted peanut flours prepared in spelt wheat). However, the detectability decreased between 10 and 1000 times in processed samples depending on the treatment. The Express PCR-bioplatform was applied to the detection of peanut traces in foodstuff.engAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/Disposable amperometric biotool for peanut detection in processed foods by targeting a chloroplast DNA markerjournal article1873-3573https://doi.org/10.1016/j.talanta.2024.126350https://www.sciencedirect.com/science/article/pii/S003991402400729Xopen access543577.1664575.1Electrochemical bioplatformChloroplast DNAPeanutExpress PCRProcessed foodsQuímica analítica (Química)Bioquímica (Biología)Genética2301 Química Analítica3309 Tecnología de Los Alimentos2409 Genética