Soriano, SagrarioCarmona, AndrésTriviño, FranciscoRodríguez, MarianoÁlvarez Benito, MarinaMartín Malo, AlejandroÁlvarez Lara, María AntoniaRamírez, RafaelAljama, PedroCarracedo Añón, Julia María2026-02-062026-02-062014-10-22Soriano, S., Carmona, A., Triviño, F., Rodriguez, M., Alvarez-Benito, M., Martín-Malo, A., Alvarez-Lara, M.-A., Ramírez, R., Aljama, P., & Carracedo, J. (2014). Endothelial damage and vascular calcification in patients with chronic kidney disease. American Journal of Physiology - Renal Physiology, 307(11), 1302-1311. https://doi.org/10.1152/ajprenal.00114.20141931-857X10.1152/ajprenal.00114.2014https://hdl.handle.net/20.500.14352/131831ACKNOWLEDGMENTS: The authors are grateful to M. J. Jimenez and R. Moyano for technical assistance. GRANTS: This work was supported by Fondo de Investigación Sanitaria, Instituto de Salud Carlos III Grants PI0900836, PI1000960, PI1101536, and PI1201489; RETICs Red Renal Grant RD0600160007; Junta de Andalucía Grants JA0797-2010, P08-CTS-3797, P010-CTS-6337, P11-CTS-7352; and Fundación Nefrológica. J. Carracedo was supported by a contract from Fundación de Investigaciones Biomédicas de Córdoba (Programa Nicolás Monardes).Vascular calcification (VC) is a frequent complication of chronic kidney disease (CKD) and is a predictor of cardiovascular morbidity and mortality. In the present study, we investigated the potential involvement of endothelial microparticles (MPs) and endothelial progenitor cells (EPCs) in the generation of VC in CKD patients. The number of circulating EMPs is greater in patients with VC than without VC (307 ± 167 vs. 99 ± 75 EMPs/μl, P < 0.001). The percentage of EPCs is significantly lower in patient with VC than in patients without VC (0.14 ± 0.11% vs. 0.25 ± 0.18%, P = 0.002). The number of EPCs expressing osteocalcin (OCN) was higher in VC patients (349 ± 63 cells/100,000) than in non-VC patients (139 ± 75 cells/100,000, P < 0.01). In vitro, MPs obtained from CKD patients were able to induce OCN expression in EPCs from healthy donors; the increase in OCN expression was more accentuated if MPs were obtained from CKD patients with VC. MPs from CKD patients also induced OCN expression in vascular smooth muscle cells and fibroblasts. In CKD patients, the rise in endothelial MPs associated with a decrease in the number of EPCs, suggesting an imbalance in the processes of endothelial damage and repair in CKD patients, mainly those with VC. Our results suggest that EPCs, through OCN expression, may directly participate in the process of VC.engjournal article1522-1466https://doi.org/10.1152/ajprenal.00114.2014restricted access616.612Chronic kidney diseaseEndothelial microparticlesEndothelial progenitor cellsVascular calcificationNefrología y urologíaBioquímica (Biología)Sistema cardiovascular3205.06 Nefrología2403 Bioquímica