Valera Arévalo, GemmaRodríguez San Pedro, María del MarCaro, Paula JaraCabanillas, VíctorOrtiz Diaz, María GabrielaFiguer, AndreaYuste, ClaudiaRamírez, RafaelAlique, MatildeMorales Ruiz, EnriqueGuerra Pérez, NataliaCarracedo Añón, Julia María2025-04-022025-04-022025Valera-Arévalo, G., Rodríguez-San Pedro, M. d. M., Caro, P. J., Cabanillas, V., Ortiz-Diaz, M. G., Figuer, A., Yuste, C., Ramírez, R., Alique, M., Morales, E., Guerra-Pérez, N., & Carracedo, J. (2025). Oxidative Score and Microvesicle Profile Suggest Cardiovascular Risk in Chronic Kidney Disease. Antioxidants, 14(2), 178. https://doi.org/10.3390/antiox140201782076-392110.3390/antiox14020178https://hdl.handle.net/20.500.14352/119159This study was funded by Instituto de Salud Carlos III through the project “PI17/01029”, “PI20/01321” and “PI23/01109” co-funded by European Regional Development Fund “A way to make Europe”. This research was also funded by grants from the Instituto de Salud Carlos III (ISCIII) and cofounded by Fondos Europeos de Desarrollo Regional (FEDER): “PI19/00240” from Universidad de Alcalá (“Ayuda de la Línea de Actuación Excelencia para el Profesorado Universitario de la UAH”; EPU-INV-UAH/2022/001) to M.A. RICORS 2040. A.F. enjoyed the PFIS contract “FI20/00018”. Also, G.V. received a contract from the Instituto de Investigacion Sanitario del Hospital Universitario 12 de Octubre (imas12) “I + 12-AY2OO414-1”.Chronic kidney disease (CKD) is associated with a high incidence of cardiovascular disease (CVD) due to the accumulation of uremic toxins, altered redox state, and chronic systemic inflammation. This study aimed to analyze the relationship between the redox status of patients with CKD and the phenotype of microvesicles (MVs) subtypes, and cardiovascular events. The oxidative stress level of each participant was determined using an individualized OXY-SCORE. The relationship between pro-oxidant and antioxidant parameters and the expression of membrane markers in endothelial-derived microvesicles (EMVs) and platelet-derived microvesicles (PMVs) was established. Patients with advanced CKD (ACKD) and hemodialysis (HD) had a higher OXY-SCORE than healthy subjects (HS), whereas peritoneal dialysis (PD) patients had similar scores to HS. PD patients showed elevated PMVs and CD41 expression, whereas HD patients had higher EMVs and CD31 expression. Patients with ACKD had higher tissue factor (TF) expression in the PMVs and EMVs. TF expression was correlated with xanthine oxidase (XO) activity and was negatively correlated with antioxidant parameters. Patients with cardiovascular events show elevated TF. In conclusion, microvesicles and oxidative stress may serve as markers of cardiovascular risk in CKD, with TF expression in PMVs and EMVs being potential predictive and prognostic biomarkers of CVD.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal articlehttps://doi.org/10.3390/antiox14020178https://www.mdpi.com/2076-3921/14/2/178open access616.1616.61577.1577.2Chronic kidney diseaseOxidative stressMicrovesiclesCardiovascular riskEndothelial dysfunctionHemodialysisPeritoneal dialysisReactive oxygenSpeciesAntioxidantsCoagulationBioquímica (Biología)Biología molecular (Biología)CardiologíaNefrología y urología2403 Bioquímica2415 Biología Molecular3205.06 Nefrología3205.01 Cardiología