Ahijado Guzmán, RubénPrasad, JanakRosman, ChristinaHenkel, AndreasTome, LydiaSchneider, DirkRivas, GermánSönnichsen, Carsten2024-01-092024-01-092014Ahijado-Guzmán, Rubén, et al. «Plasmonic Nanosensors for Simultaneous Quantification of Multiple Protein–Protein Binding Affinities». Nano Letters, vol. 14, n.o 10, octubre de 2014, pp. 5528-32. https://doi.org/10.1021/nl501865p.1556-276X10.1021/nl501865phttps://hdl.handle.net/20.500.14352/92112Most of current techniques used for the quantification of protein–protein interactions require the analysis of one pair of binding partners at a time. Herein we present a label-free, simple, fast, and cost-effective route to characterize binding affinities between multiple macromolecular partners simultaneously, using optical dark-field spectroscopy and individual protein-functionalized gold nanorods as sensing elements. Our NanoSPR method could easily become a simple and standard tool in biological, biochemical, and medical laboratories.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://doi.org/10.1021/nl501865prestricted access544NanoSPRPlasmonic nanosensorsMultiple protein−protein interactionsOptical dark-field spectroscopyFtsZQuímica física (Química)2307 Química Física