Balas, DeliaFernández Moreira, EstebanCampa, Adela G. de la2025-10-152025-10-151998-06-01Balas D, Fernández-Moreira E, De La Campa AG. 1998. Molecular Characterization of the Gene Encoding the DNA Gyrase A Subunit of Streptococcus pneumoniae. J Bacteriol 180:. https://doi.org/10.1128/jb.180.11.2854-2861.19980021-919310.1128/JB.180.11.2854-2861.1998https://hdl.handle.net/20.500.14352/124943The gene encoding the DNA gyrase A subunit of Streptococcus pneumoniae was cloned and sequenced. The gyrA gene codes for a protein of 822 amino acids homologous to the gyrase A subunit of eubacteria. Translation of the gene in an Escherichia coli expression system revealed a 92-kDa polypeptide. A sequence-directed DNA curvature was identi®ed in the promoter region of gyrA. The bend center was mapped and located between the 35 and 10regions of the promoter. Primer extension analysis showed that gyrA transcription initiates 6 bp downstream of an extended 10 promoter. The possible implications of the bent DNA region as a regulatory element in the transcription of gyrA are discussed.engjournal article1098-5530https://doi.org/10.1128/jb.180.11.2854-2861.1998https://journals.asm.org/doi/10.1128/jb.180.11.2854-2861.1998?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub++0pubmedrestricted access577.2DNA topoisomerase IVGyrase AStreptococcus pneumoniaeCiencias Biomédicas32 Ciencias Médicas