Sánchez Ramos, CeliaBonnin Arias, Cristina NataliaGuerrera, María CristinaGarcía Calavia, MartaChamorro Gutiérrez, EvaMontalbano, GiuseppeLópez Velasco, SalvadorLópez Muñiz, Alfonso JoaquínGermanà, AntoninoVega Álvarez, José Antonio2024-02-122024-02-122012Sánchez-Ramos C, Bonnin-Arias C, Guerrera MC, Calavia MG, Chamorro E, Montalbano G, López-Velasco S, López-Muñiz A, Germanà A, Vega JA. Light regulates the expression of the BDNF/TrkB system in the adult zebrafish retina. Microsc Res Tech. 2013 Jan;76(1):42-9. doi: 10.1002/jemt.22133. Epub 2012 Oct 16. PMID: 23070877.1059-910X10.1002/jemt.22133https://hdl.handle.net/20.500.14352/101320The retina of the adult zebrafish express brain‐derived neurotrophic factor (BDNF) and its signaling receptor TrkB. This functional system is involved in the biology of the vertebrate retina and its expression is regulated by light. This study was designed to investigate the effects of cyclic (12 h light/12 h darkness) or continuous (24 h) exposure during 10 days to white light, white‐blue light, and blue light, as well as of darkness, on the expression of BDNF and TrkB in the retina. BDNF and TrkB were assessed in the retina of adult zebrafish using quantitative real‐time polymerase chain reaction and immunohistochemistry. Exposure to white, white‐blue, and blue light causes a decrease of BDNF mRNA and of BDNF immunostaining, independently of the pattern of light exposition. Conversely, in the same experimental conditions, the expression of TrkB mRNA was upregulated and TrkB immunostaining increased. Exposition to darkness diminished BDNF and TrkB mRNAs, and abolished the immunostaining for BDNF but not modified that for TrkB. These results demonstrate the regulation of BDNF and TrkB by light in the retina of adult zebrafish and might contribute to explain some aspects of the complex pathophysiology of light‐induced retinopathies.engjournal article1097-0029http://dx.doi.org/10.1002/jemt.2213323070877https://pubmed.ncbi.nlm.nih.gov/23070877/https://digibuo.uniovi.es/dspace/handle/10651/5960https://abacus.universidadeuropea.com/handle/11268/719restricted access612.84617.735611.843612.019RetinaZebrafishLightDarknessTrkBBrain-derived neurotrophic factorCiencias BiomédicasFisiología animal (Biología)Óptica fisiológica3299 Otras Especialidades Médicas2401.13 Fisiología Animal2209.24 Física de la Visión2411.15 Fisiología de la Visión