Juárez Martín-Delgado, IgnacioToro Fernández, Juan FranciscoVaquero Yuste, ChristianMolina Alejandre, MartaLasa, InmaculadaGómez, RemediosLópez, AdelaMartín Villa, José ManuelGutiérrez, Alberto2023-06-172023-06-172021-06-10Juárez Martín-Delgado, I., Toro Fernández, J. F., Vaquero Yuste, C. et al. «A Reliable and Standardizable Differential PCR and qPCR Methodology Assesses HER2 Gene Amplification in Gastric Cancer». Biology, vol. 10, n.o 6, junio de 2021, p. 516. DOI.org (Crossref), https://doi.org/10.3390/biology10060516.2079-773710.3390/biology10060516https://hdl.handle.net/20.500.14352/6851We have applied two PCR techniques, differential PCR (diffPCR) and qPCR for the identification of HER2 gene amplifications in genomic DNA of tumor and distal gastric samples from patients with gastric cancer. The diffPCR technique consists of the simultaneous amplification of the HER2 gene and a housekeeping gene by conventional PCR and the densitometric analysis of the bands obtained. We established a cut-off point based on the mean and standard deviation analyzing the DNA of 30 gastric tissues from patients undergoing non-cancer gastrectomy. diffPCR and qPCR yielded consistent results. HER2-overexpression was detected in 25% of patients and was further confirmed by immunohistochemistry and immunofluorescence. The approaches herein described may serve as complementary and reliable methods to assess HER2 amplification.engAtribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/journal articlehttps://doi.org/10.3390/biology10060516https://www.mdpi.com/2079-7737/10/6/516/htmopen accessHER2Gastric cancerCancerPCRImmunotherapyGenética médicaInmunologíaOncologíaBiología molecular (Biología)2410.07 Genética Humana2412 Inmunología3201.01 Oncología2415 Biología Molecular