Vicente Valor, JuanTesolato, Sofía ElenaPaz Cabezas, MateoGómez Garre, María Dulce NombreOrtega Hernández, AdrianaDe la Serna Esteban, Sofía CristinaDomínguez Serrano, María InmaculadaDziakova, JanaRivera Alonso, DanielJarabo Sarceda, José RamónGómez Martínez, Ana MaríaHernando Trancho, FlorentinoTorres García, Antonio JoséIniesta Serrano, María Pilar2025-02-062025-02-062025-01-16Vicente-Valor, J.; Tesolato, S.; Paz-Cabezas, M.; Gómez-Garre, D.; Ortega-Hernández, A.; de la Serna, S.; Domínguez-Serrano, I.; Dziakova, J.; Rivera, D.; Jarabo, J.-R.; et al. Fecal Microbiota Strongly Correlates with Tissue Microbiota Composition in Colorectal Cancer but Not in Non-Small Cell Lung Cancer. Int. J. Mol. Sci. 2025, 26, 717. https:// doi.org/10.3390/ijms260207171661-659610.3390/ijms26020717https://hdl.handle.net/20.500.14352/117864Microbiota could be of interest in the diagnosis of colorectal and non-small cell lung cancer (CRC and NSCLC). However, how the microbial components of tissues and feces reflect each other remains unknown. In this work, our main objective is to discover the degree of correlation between the composition of the tissue microbiota and that of the feces of patients affected by CRC and NSCLC. Specifically, we investigated tumor and non-tumor tissues from 38 recruited patients with CRC and 19 with NSCLC. DNA from samples was submitted for 16S rDNA metagenomic sequencing, followed by data analysis through the QIIME2 pipeline and further statistical processing with STATA IC16. Tumor and non-tumor tissue selected genera were highly correlated in both CRC and NSCLC (100% and 81.25%). Following this, we established tissue–feces correlations, using selected genera from a LEfSe analysis previously published. In CRC, we found a strong correlation between the taxa detected in feces and those from colorectal tissues. However, our data do not demonstrate this correlation in NSCLC. In conclusion, our findings strongly reinforce the utility of fecal microbiota as a non-invasive biomarker for CRC diagnosis, while highlighting critical distinctions for NSCLC. Furthermore, our data demonstrate that the microbiota components of tumor and non-tumor tissues are similar, with only minor differences being detected.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal article1422-0067https://doi.org/10.3390/ijms2602071739859429https://www.mdpi.com/1422-0067/26/2/717https://pubmed.ncbi.nlm.nih.gov/39859429/open access616-006.04MicrobiotaColorectal cancerNon-small cell lung cancerCiencias Biomédicas24 Ciencias de la Vida32 Ciencias Médicas