Anadón Navarro, Arturo RamónSuarez, FrankMartínez Caballero, María AranzazuCastellano Santos, Víctor JesúsMartínez Caballero, MartaAres Lombán, IrmaRamos Alonso, EvaGamboa, FelipeMartínez Larrañaga, María Rosa2024-02-012024-02-012011Anadón A, Suárez FH, Martínez MA, Castellano V, Martínez M, Ares I, Ramos E, Gamboa F, Martínez-Larrañaga MR. Plasma disposition and tissue depletion of difloxacin and its metabolite sarafloxacin in the food producing animals, chickens for fattening. Food Chem Toxicol. 2011 Feb;49(2):441-9. doi: 10.1016/j.fct.2010.11.021. Epub 2010 Nov 23. PMID: 21108984.0278-691510.1016/j.fct.2010.11.021https://hdl.handle.net/20.500.14352/97929Chickens were used to investigate plasma disposition of difloxacin after single intravenous (IV) and oral dose (10 mg/kg body weight (BW)) and to study residue depletion of difloxacin and its major metabolite sarafloxacin after multiple oral doses (10 mg difloxacin/kg BW, daily for 5 days). Plasma and tissue samples were analyzed using a HPLC method. After IV and oral administration, plasma drug concentration-time curves were best described by a two-compartment open model. Mean (± SD) elimination half-lives (t(½)β) of difloxacin were 9.53±1.00 and 12.23±1.81 h after IV and oral administration. Maximum plasma concentration was 2.34±0.50 μg/ml and interval from oral administration until maximal concentration was 1.34±0.03 h. Oral bioavailability was found to be 68.89±15.21%. Difloxacin was converted to sarafloxacin. After multiple oral dose (10mg difloxacin/kg BW, daily for 5 days), mean kidney, liver, muscle and skin + fat tissue concentrations of difloxacin and sarafloxacin ranging between 604.8±132.5 and 368.1±52.5 μg/kg and 136.4±18.3 and 10.4±1.2 μg/kg, respectively, were measured 1 day after administration of the final dose of difloxacin. A withdrawal time of 5 days was necessary to ensure that the residues of difloxacin were less than the maximal residue limits (MRL) or tolerance established by the European Union.engjournal article1873-6351https://doi.org/10.1016/j.fct.2010.11.02121108984restricted accessDifloxacinSarafloxacinPlasma and tissue depletionWithdrawal timeChickens for fatteningCiencias Biomédicas3214 Toxicología