Vivero López, MaríaSparacino, ChiaraQuelle Regaldie, AnaSánchez Piñón, Laura ElenaCandal Suárez, EvaBarreiro Iglesias, AntónHuete Toral, FernandoCarracedo Rodríguez, Juan GonzaloOtero Casal, Ana MaríaConcheiro Nine, ÁngelAlvarez Lorenzo, Carmen2023-06-222023-06-222022-10-130378-517310.1016/j.ijpharm.2022.122281https://hdl.handle.net/20.500.14352/73272Received 10 July 2022; Received in revised form 3 October 2022; Accepted 7 October 2022Ocular health may strongly benefit from the supply of antioxidant agents that counteract free radicals and reactive oxygen species responsible for long-term eye diseases. Additionally, natural antioxidants like resveratrol can inhibit bacteria growth and restore natural microbiota. However, their use is hindered by limited solubility, fast degradation, and low ocular permeability. This work aimed to overcome these limitations by preparing single and mixed micelles of Pluronic® F127 and casein that serve as resveratrol nanocarriers. Single and mixed (0.1 % casein) micelles (0.0 to −17.0 mV; 2.4 to 32.7 nm) increased 50-fold resveratrol solubility, remained stable for one month at 4 °C, withstood fast dilution, underwent sol-to-gel transitions in the 23.9–27.1 °C range, and exhibited potent antioxidant properties. All formulations successfully passed the HET-CAM assay but showed Pluronic®-casein dose-dependent toxicity in the zebrafish embryo model. Resveratrol-loaded single and mixed micelles (10–15 mM Pluronic® F127) displayed antimicrobial activity against S. aureus and P. aeruginosa. The micelles favored resveratrol accumulation in cornea and sclera, but mixed micelles showed larger lag times and provided lower amount of resveratrol permeated through sclera. In vivo (rabbit) tests confirmed the safety of resveratrol-loaded single micelles and their capability to supply resveratrol to anterior and posterior eye segments.engAtribución-NoComercial-SinDerivadas 3.0 Españahttps://creativecommons.org/licenses/by-nc-nd/3.0/es/Pluronic®/casein micelles for ophthalmic delivery of resveratrol: In vitro, ex vivo, and in vivo testsjournal articlehttps://doi.org/10.1016/j.ijpharm.2022.122281https://www.sciencedirect.com/science/article/pii/S0378517322008365open access547:617.7Mixed micellesResveratrolPluronic® F127ZebrafishIn vivo tear fluidlevelsOcular biodistributionBioquímica (Medicina)Oftalmología3201.09 Oftalmología