Cantero Bahíllo, EmmaSiles Sánchez, María de las NievesJaime, LauraMartín, DianaNavarro Del Hierro, Joaquín2025-02-102025-02-102024-10-10Cantero-Bahillo, E., Siles-Sánchez, M. d. l. N., Jaime, L., Martin, D., & Navarro del Hierro, J. (2024). Ultrasound-assisted Co-extraction and characterization of saponin-rich Co-extracts from fenugreek seeds (Trigonella foenum-graecum L.) and quinoa husk (Chenopodium quinoa Willd.) and their hydrolysates. Food Bioscience, 62. https://doi.org/10.1016/J.FBIO.2024.1052752212-429210.1016/j.fbio.2024.105275https://hdl.handle.net/20.500.14352/117966Author contributions: Emma Cantero-Bahillo: Writing – original draft, Methodology, Investigation, Formal analysis, Data curation, Conceptualization. María de las Nieves Siles-Sánchez: Methodology, Investigation, Formal analysis, Data curation. Laura Jaime: Writing – review & editing, Supervision, Project administration, Funding acquisition. Diana Martin: Writing – review & editing, Supervision, Project administration, Funding acquisition, Conceptualization. Joaquín Navarro del Hierro: Writing – review & editing, Supervision, Conceptualization.The co-extraction of steroidal and triterpenoid saponins, followed by hydrolysis to produce sapogenin-rich extracts, offers an efficient strategy to enhance the multi-bioactive potential of extracts. The resulting chemical composition modifications, including the presence of other co-extracted or co-hydrolyzed compounds, must also be considered. In this study, ultrasound-assisted co-extraction of fenugreek seeds and quinoa husk using methanol and 50% aqueous methanol was conducted to obtain extracts and co-extracts with varying proportions of steroidal and triterpenoid saponins, in ratios of 0:100, 25:75, 50:50, 75:25, and 100:0, respectively. Aqueous methanol produced the highest extraction yields (15–25%) and saponin contents (40–70%) in the extracts and co-extracts. The conversion by microwave-assisted acid hydrolysis of saponins to sapogenins was more efficient for triterpenoid saponins, leading to a higher triterpenoid sapogenin content in all co-extracts, accounting for over 70% of the total sapogenins. The co-extracts also contained other bioactive compounds, such as phenolic compounds (3–4 g/100 g), with their content being favored by the presence of fenugreek. Hydrolysis altered the overall composition of the saponin-rich co-extracts, enriching the resulting hydrolyzed products in both types of sapogenins, along with fatty acids, carbonyls, non-protein nitrogen compounds and organic acids, while reducing monoglycerides, removing carbohydrates and significantly modifying the phenolic profile by eliminating many original phenolic compounds and generating new ones. Therefore, co-extraction and post-extraction hydrolysis of fenugreek and quinoa allowed the combination of triterpenoid and steroidal saponins or sapogenins into single products, along with other specific compounds from both sources.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal articlehttps://doi.org/10.1016/j.fbio.2024.105275open access636.09Co-extractionSaponinsSapogeninsPhenolic compoundsMicrowave-assisted acid hydrolysisUltrasound-assisted extractionCiencias Biomédicas24 Ciencias de la Vida