Luna, CarlosCarmona, AndrésAlique, MatildeCarracedo Añón, Julia MaríaRamírez, Rafael2026-02-032026-02-032015-12-22Luna C, Carmona A, Alique M, Carracedo J and Ramirez R (2015) TNFα-Damaged-HUVECs Microparticles Modify Endothelial Progenitor Cell Functional Activity. Front. Physiol. 6:395. doi: 10.3389/fphys.2015.003951664-042X10.3389/fphys.2015.00395https://hdl.handle.net/20.500.14352/131422This work was supported by Plan Nacional Proyectos de Investigación en Salud of Instituto de Salud Carlos III (ISCIII) Fondos FEDER Grants (PI11/01536, PI12/01489, PI14/00806, PI10/00960 and RD12/0021/0011); Junta de Andalucía Grants JA0797-2010, P010-CTS-6337, P11-CTS-7352. JC was supported by a contract from Fundación de Investigaciones Biomédicas de Córdoba (Programa Nicolás Monardes). CL and AC are fellows from Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía (CTS-6337). MA is supported by “Ayuda Postdoctoral Programa Propio” from Universidad de Alcalá.Endothelial progenitor cells (EPCs) have an important role in the maintenance of vascular integrity and homeostasis. While there are many studies that explain EPCs mechanisms action, there are few studies that demonstrate how they interact with other emerging physiological elements such as Endothelial Microparticles (EMPs). EMPs are membranous structures with a size between 100 and 1000 nm that act as molecular information transporter in biological systems and are known as an important elements in develop different pathologies; moreover a lot of works explains that are novel biomarkers. To elucidate these interactions, we proposed an in vitro model of endothelial damage mediated by TNFalpha, in which damaged EMPs and EPCs are in contact to assess EPCs functional effects. We have observed that damaged EMPs can modulate several EPCs classic factors as colony forming units (CFUs), contribution to repair a physically damaged endothelium (wound healing), binding to mature endothelium, and co-adjuvants to the formation of new vessels in vitro (angiogenesis). All of these in a dose-dependent manner. Damaged EMPs at a concentration of 103 MPs/ml have an activating effect of these capabilities, while at concentrations of 105 MPs/ml these effects are attenuated or reduced. This in vitro model helps explain that in diseases where there is an imbalance between these two elements (EPCs and damaged EMPs), the key cellular elements in the regeneration and maintenance of vascular homeostasis (EPCs) are not fully functional, and could explain, at least in part, endothelial dysfunction associated in various pathologies.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal articlehttps://doi.org/10.3389/fphys.2015.00395https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2015.00395/fullopen access612.1616.1616.15576Endothelial microparticlesEndothelial progenitor cellsAngiogenesisTNFalphaHUVECsFisiologíaSistema cardiovascularHematologíaBiología celular (Biología)2411.03 Fisiología Cardiovascular3207.04 Patología Cardiovascular3207.08 Hematología2410.03 Citología Humana