Vargas Balbuena, JavierGómez Pedrero, José AntonioQuiroga Mellado, Juan AntonioAlonso Fernández, José2023-06-222023-06-222022-01-311094-408710.1364/OE.444675https://hdl.handle.net/20.500.14352/71328© 2022 Optica Publishing Group. Funding. Ministerio de Ciencia e Innovación (PID2019-108850RA-I00).We present an approach to enhance cryo-electron microscopy (cryo-EM) postprocessed maps based on a multiscale tubular filter. The method determines a tubularness measure locally by the analysis of the eigenvalues of the Hessian matrix. This information is used to enhance elongated local structures and to attenuate blob-like and plate-like structures. The approach, thus, introduces a priori information in the reconstructions to improve their interpretability and analysis at high-resolution. The proposed method has been tested with simulated and real cryo-EM maps including recent reconstructions of the SARS-CoV-2. Our results show that our methods can improve obtained reconstructions.engAtribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/journal articlehttp://dx.doi.org/10.1364/OE.444675https://opg.optica.orgopen access535ResolutionÓptica (Física)2209.19 Óptica Física