Cáceres Ramos, Sara CristinaMonsalve, BeatrizPeña Fernández, Laura LuisaAndrés Gamazo, Paloma Jimena deAlonso‐Diez, ÁngelaIllera del Portal, Josefina MaríaWoodward, WendyReuben, JamesSilván Granado, GemaIllera Del Portal, Juan Carlos2024-01-252024-01-252017Caceres S, Monsalve B, Peña L, de Andres PJ, Alonso-Diez A, Illera MJ, Woodward WA, Reuben JM, Silvan G, Illera JC. In vitro and in vivo effect of flutamide on steroid hormone secretion in canine and human inflammatory breast cancer cell lines. Vet Comp Oncol. 2018 Mar;16(1):148-158. doi: 10.1111/vco.12324. Epub 2017 Jun 6. PMID: 28589573.1476-581010.1111/vco.12324https://hdl.handle.net/20.500.14352/95543The aim was to study the effects of flutamide on cell proliferation, in vivo tumour growth andsteroid production in canine and human IBC cell lines. IPC-366 and SUM149 cell cultures wereexposed to flutamide concentrations for 72 hours. Additionally, IPC-366 and SUM149 xeno-transplanted mice were treated subcutaneously with flutamide 3 times a week for 2 weeks.Steroid hormones determination in culture media, serum and tumour homogenates (pregneno-lone, progesterone, androstenedione, testosterone, dihydrotestosterone, 17β-oestradiol andoestrone sulphate) were assayed by EIA. in vitro cell proliferation percentages showed adecrease in all flutamide dosages in IPC-366 and SUM149. in vivo flutamide reduced tumoursize by 55% to 65%, and metastasis rates decreased. In treated groups, androgen levels in cul-ture media, serum and tumour homogenates were increased as oestrogen levels decreased. These results suggest that flutamide treatment inhibits cell proliferation and promotes tumourreduction by increasing androgen levels and also support future therapy approachesengAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://www.doi.org/10.1111/vco.1232428589573https://pubmed.ncbi.nlm.nih.gov/28589573/https://www.scopus.com/record/display.uri?eid=2-s2.0-85020217791&origin=resultslisthttps://onlinelibrary.wiley.com/doi/10.1111/vco.12324restricted access61IBCIMCFlutamideSteroid hormonesTumour growthCiencias Biomédicas32 Ciencias Médicas