Romero Picó, AmparoGarrido Novelle, MartaFolgueira, CintiaLópez, MiguelNogueiras, RubénDiéguez, Carlos2024-01-232024-01-232013Romero-Picó, A., Novelle, M.G., Folgueira, C. et al. Central manipulation of dopamine receptors attenuates the orexigenic action of ghrelin. Psychopharmacology 229, 275–283 (2013). https://doi.org/10.1007/s00213-013-3096-70033-315810.1007/s00213-013-3096-7https://hdl.handle.net/20.500.14352/94910This work has been supported by grants from Ministerio de Educacion y Ciencia (CD: BFU2011; ML: RyC-2007-00211; RN: RYC-2008-02219 and BFU2012-35255), Xunta de Galicia (ML: 10PXIB208164PR and RN: EM 2012/039 and 2012-CP069), Fondo Investigationes Sanitarias (ML: PS09/01880), Centro de Investigación Biomédica en Red (CIBER) de Fisiopatología de la Obesidad y Nutrición (CIBERobn). CIBERobn is an initiative of the Instituto de Salud Carlos III (ISCIII) of Spain which is supported by FEDER funds. The research leading to these results has also received funding from the European Community's Seventh Framework Programme under the following grants (CD, ML and RN: FP7/2007-2013: no. 245009: NeuroFAST, and RN: ERC-2011-StG-OBESITY53-281408).Objective Recent evidence suggests that ghrelin, a peptidic hormone stimulating food intake, interacts with the dopamine signaling. This interaction has been demonstrated to modulate several effects of ghrelin, such as locomotor activity, memory, and food intake. Ghrelin increases dopamine levels in the shell of the nucleus accumbens stimulating food intake, while ablation of the ghrelin receptor attenuates the hypophagia caused by the activation of dopamine receptor 2. However, it is not known whether the orexigenic action of ghrelin is due to changes in central dopamine receptors. Materials and methods We used Sprague–Dawley rats injected with different dopamine receptor agonists, antagonists, and ghrelin. Results We demonstrate that the specific central blockade of dopamine receptor 1, 2, and 3 (D1, D2, and D3, respectively) reduces the orexigenic action of ghrelin. Similarly, specific central stimulation, either singly of dopamine receptor 1 or dopamine receptors 2 and 3 simultaneously, causes a significant decrease in ghrelin-induced food intake. Co-stimulation of all three receptors (D1, D2, and D3) also led to a marked attenuation in ghrelin-induced food intake. Importantly, the reduction in ghrelin-induced feeding was not caused by malaise or any type of behavioral alteration. Conclusion Taken together, these data indicate that dopamine receptors play an important role in acute stimulation of feeding behavior induced by central injection of ghrelin.engjournal article1432-2072https://doi.org/10.1007/s00213-013-3096-7restricted access577.17AppetiteDopamine receptorFood intakeBioquímica (Biología)Dietética y nutrición (Medicina)2411.04 Fisiología Endocrina2403 Bioquímica