Immobilized enzyme reactors based on nucleoside phosphorylases and 2′-deoxyribosyltransferase for the in-flow synthesis of pharmaceutically relevant nucleoside analogues

Rinaldi, FrancescaFernández-Lucas, JesúsFuente, Diego de laZheng, ChangpingBavaro, TeodoraPeters, BenjaminMassolini, GabriellaAnnunziata, FrancescaConti, PaolaMata, Isabel de laTerreni, MarcoCalleri, Enrica2023-06-162023-06-162020-03-260960-852410.1016/j.biortech.2020.123258https://hdl.handle.net/20.500.14352/6667In this work, a mono- and a bi-enzymatic analytical immobilized enzyme reactors (IMERs) were developed as prototypes for biosynthetic purposes and their performances in the in-flow synthesis of nucleoside analogues of pharmaceutical interest were evaluated. Two biocatalytic routes based on nucleoside 2′-deoxyribosyltransferase from Lactobacillus reuteri (LrNDT) and uridine phosphorylase from Clostridium perfrigens (CpUP)/purine nucleoside phosphorylase from Aeromonas hydrophila (AhPNP) were investigated in the synthesis of 2′-deoxy, 2′,3′- dideoxy and arabinonucleoside derivatives. LrNDT-IMER catalyzed the synthesis of 5-fluoro-2′-deoxyuridine and 5-iodo-2′-deoxyuridine in 65–59% conversion yield, while CpUP/AhPNP-IMER provided the best results for the preparation of arabinosyladenine (60% conversion yield).engImmobilized enzyme reactors based on nucleoside phosphorylases and 2′-deoxyribosyltransferase for the in-flow synthesis of pharmaceutically relevant nucleoside analoguesjournal articlehttps://www.sciencedirect.com/science/article/pii/S0960852420305290restricted access577.15BiocatalysisImmobilized enzyme reactorsNucleoside analoguesNucleoside 2′-deoxyribosyltransferasesNucleoside phosphorylasesBioquímica (Biología)2302 Bioquímica