Leite Fernandes, Vitor SamuelFernandes Ribeiro, Ana SofíaMartínez, María PilarOrensanz, Luis M.Barahona Gomáriz, María VictoriaMartínez-Sáenz, AnaRecio Visedo, María PazBenedito Castellote, SaraBustamante, SalvadorCarballido, JoaquínGarcía Sacristán, AlbinoPrieto Ocejo, DoloresHernández Rodríguez, Medardo Vicente2024-02-082024-02-082013-04-010022-53471527-379210.1016/j.juro.2012.10.006https://hdl.handle.net/20.500.14352/100326Purpose: We investigated the possible involvement of H2S in nitric oxide independent inhibitory neurotransmission to the pig bladder neck. Materials and methods: We used immunohistochemistry to determine the expression of the H2S synthesis enzymes cystathionine γ-lyase and cystathionine β-synthase. We also used electrical field stimulation and myographs for isometric force recordings to study relaxation in response to endogenously released or exogenously applied H2S in urothelium denuded, phenylephrine precontracted bladder neck strips under noradrenergic, noncholinergic, nonnitrergic conditions. Results: Cystathionine γ-lyase and cystathionine β-synthase expression was observed in nerve fibers in the smooth muscle layer. Cystathionine γ-lyase and cystathionine β-synthase immunoreactive fibers were also identified around the small arteries supplying the bladder neck. Electrical field stimulation (2 to 16 Hz) evoked frequency dependent relaxation, which was decreased by DL-propargylglycine and abolished by tetrodotoxin (blockers of cystathionine γ-lyase and neuronal voltage gated Na(+) channels, respectively). The cystathionine β-synthase inhibitor O-(carboxymethyl)hydroxylamine did not change nerve mediated responses. The H2S donor GYY4137 (0.1 nM to 10 μM) induced potent, concentration dependent relaxation, which was not modified by neuronal voltage gated Na(+) channels, or cystathionine γ-lyase or cystathionine β-synthase blockade. Conclusions: Results suggest that endogenous H2S synthesized by cystathionine γ-lyase and released from intramural nerves acts as a powerful signaling molecule in nitric oxide independent inhibitory transmission to the pig bladder neck.engjournal articlehttps://doi.org/10.1016/j.juro.2012.10.006restricted access591.1612.019Urinary bladderHydrogen sulfideSynaptic transmissionElectric stimulationCystathionine gamma-lyaseCiencias Biomédicas2411 Fisiología Humana