Larrosa Pérez, María Del MarBurgos-Edwards, AMartín-Pérez, LJiménez-Aspee, FTheoduloz, CSchmeda-Hirschmann, G2025-01-202025-01-202019-08-01Burgos-Edwards A, Martín-Pérez L, Jiménez-Aspee F, Theoduloz C, Schmeda-Hirschmann G, Larrosa M. Anti-inflammatory effect of polyphenols from Chilean currants (Ribes magellanicum and R. punctatum) after in vitro gastrointestinal digestion on Caco-2 cells: Anti-inflammatory activity of in vitro digested Chilean currants. J Funct Foods. 2019;59:329-36. doi:10.1016/j.jff.2019.06.00710.1016/j.jff.2019.06.007https://hdl.handle.net/20.500.14352/115014The polyphenol-enriched extracts (PEEs) of Chilean currants Ribes magellanicum and R. punctatum were submitted to in vitro gastro-intestinal digestion and their anti-inflammatory activities were assessed using differentiated human Caco-2 (clone C2BBe1) cells stimulated with interleukin 1β (IL-1 β). The inhibitory effect of non-digested and digested PEEs towards human cyclooxygenase 1 (COX-1) and COX-2, and the gene expression of COX-2 and inducible nitric oxide synthase (iNOS) was also evaluated. The digested PEE from R. punctatum decreased the secretion of IL-8, IL-6, and TNF-α; whereas R. magellanicum reduced IL-6 and TNF-α in the Caco-2 cells (p < 0.05). Both digested extracts significantly down-regulated the mRNA expression of COX-2 and iNOS (p < 0.05). PEEs showed 60% of inhibition towards COXs, with higher inhibition against COX-2. The PEEs from R. punctatum displayed better anti-inflammatory activity in all the experiments. Our results suggest that R. magellanicum and R. punctatum might be useful against intestine inflammatory conditions.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://doi.org/10.1016/j.jff.2019.06.007open access615.322Ribes sppIntestinal inflammationCOX-2iNOSinterleukin secretionMedicamentos32 Ciencias Médicas