Ochoa Hueso, RaúlParadela, CristinaPérez Corona, María EstherManrique Reol, EstebanSutton, Mark A.Mason, Kate E.Sheppard, Lucy J.Sverdrup, HaraldHaeuber, RichardHicks, W. Kevin2025-12-152025-12-152014Ochoa-Hueso, R., Paradela, C., Pérez-Corona, M., Manrique, E. (2014). Pigment Ratios of the Mediterranean Bryophyte Pleurochaete squarrosa Respond to Simulated Nitrogen Deposition. In: Sutton, M., Mason, K., Sheppard, L., Sverdrup, H., Haeuber, R., Hicks, W. (eds) Nitrogen Deposition, Critical Loads and Biodiversity. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7939-6_23978-94-007-7938-910.1007/978-94-007-7939-6_23https://hdl.handle.net/20.500.14352/129034Proceedings of the International Nitrogen Initiative Workshop, linking experts of the Convention on Long-Range Transboundary Air Pollution and the Convention on Biological Diversity.Nitrogen (N) deposition alters ecosystem structure and functioning. To study potential impacts on Mediterranean ecosystems, we designed a field fertilization experiment where NH4NO3 was added at four rates (0, 10, 20 and 50 kg N ha−1 year−1). The terricolous moss Pleurochaete squarrosa was sampled and pigments extracted in spring-autumn 2008 and spring 2009. Simulated N deposition increased lutein and VAZ pigments to chlorophyll ratios in autumn 2008; β-carotene to chlorophyll ratio was reduced in spring 2009; (neoxantin + lutein) to β-carotene increased with N supply and this was explained as higher investment in light-harvesting complexes than in reaction centres. Response of carotene to chlorophylls and of (neoxantin + lutein)/β-carotene to N enrichment were only evident when soil NH4 and Mn, respectively, were used as covariates. Thus, covariance analyses are highly recommended to detect N fertilization effects on terricolous species when field experiments are set up in highly heterogeneous environments.engbook part978-94-007-7939-6https://doi.org/10.1007/978-94-007-7939-6_23https://link.springer.com/chapter/10.1007/978-94-007-7939-6_23#citeasmetadata only access581.1581.5582.32BryophyteMediterraneanNitrogen depositionPigment ratiosEcología (Biología)Fisiología vegetal (Biología)Medio ambiente natural2417.13 Ecología Vegetal2417.19 Fisiología Vegetal2417.91 Flora Mediterránea