Dysregulation of prostaglandine E2 and BDNF signaling mediated by estrogenic dysfunction induces primary hippocampal neuronal cell death after single and repeated paraquat treatment

Moyano-Cires Ivanoff, Paula VivianaSanjuan, JavierGarcía Sánchez, José ManuelAnadón Baselga, María JoséNaval López, María VictoriaSola Vendrell, EmmaGarcía Lobo, JimenaFrejo Moya, María TeresaPino Sans, Javier Del2025-01-282025-01-282020-10-01Moyano, P., Sanjuan, J., García, J. M., Anadon, M. J., Naval, M. V., Sola, E., ... & Del Pino, J. (2020). Dysregulation of prostaglandine E2 and BDNF signaling mediated by estrogenic dysfunction induces primary hippocampal neuronal cell death after single and repeated paraquat treatment. Food and Chemical Toxicology, 144, 111611.0278-691510.1016/j.fct.2020.111611https://hdl.handle.net/20.500.14352/116626Author contributions: Paula Moyano: Methodology, Investigation, Writing - original draft, Writing - review & editing. Javier Sanjuan: Methodology, Investigation, Writing - original draft, Writing - review & editing. José Manuel García: Methodology, Investigation, Validation, Writing - review & editing. María José Anadon: Data curation, Writing - review & editing. Maria Victoria Naval: Formal analysis, Data curation. Emma Sola: Investigation, Visualization. Jimena García: Investigation, Validation. María Teresa Frejo: Formal analysis, Writing - review & editing. Javier del Pino: Conceptualization, Methodology, Project administration, Supervision, Writing - review & editing.Paraquat (PQ) produces hippocampal neuronal cell death and cognitive dysfunctions after unique and continued exposure, but the mechanisms are not understood. Primary hippocampal wildtype or βAPP-Tau silenced cells were co-treated with PQ with or without E2, N-acetylcysteine (NAC), NS-398 (cyclooxygenase-2 inhibitor), MF63 (PGES-1 inhibitor) and/or recombinant brain-derived neurotrophic factor (BDNF) during one- and fourteen-days to studied PQ effect on prostaglandin E2 (PGE2) and BDNF signaling and their involvement in hyperphosphorylated Tau (pTau) and amyloid-beta (Aβ) protein formation, and oxidative stress generation, that lead to neuronal cell loss through estrogenic disruption, as a possible mechanism of cognitive dysfunctions produced by PQ. Our results indicate that PQ overexpressed cyclooxygenase-2 that leads to an increase of PGE2 and alters the expression of EP1-3 receptor subtypes. PQ induced also a decrease of proBDNF and mature BDNF levels and altered P75NTR and tropomyosin receptor kinase B (TrkB) expression. PQ induced PGE2 and BDNF signaling dysfunction, mediated through estrogenic disruption, leading to Aβ and pTau proteins synthesis, oxidative stress generation and finally to cell death. Our research provides relevant information to explain PQ hippocampal neurotoxic effects, indicating a probable explanation of the cognitive dysfunction observed and suggests new therapeutic strategies to protect against PQ toxic effects.engDysregulation of prostaglandine E2 and BDNF signaling mediated by estrogenic dysfunction induces primary hippocampal neuronal cell death after single and repeated paraquat treatmentjournal articlehttps://doi.org/10.1016/j.fct.2020.111611restricted access615.9616.8Hippocampal neuronsParaquatAβ and Tau proteinsEROxidative stressCell deathPGE2BDNFToxicología (Medicina)Neurociencias (Medicina)3214 Toxicología3205.07 Neurología